中文摘要：本研究应用定量蛋白质组学技术（iTRAQ）分析了5位顽固性肺炎支原体肺炎（RMPP）患者、5位非顽固性支原体肺炎患者（NRMPP）和5位健康儿童血液中MP-相关的蛋白质。研究通过系统关系和Cytoscape分析获得了蛋白质注释信息，基于这些信息对肺炎支原体相关的蛋白进行功能分类、亚细胞定位和蛋白互作网络分析。研究从RMPP和NRMPP组鉴定出260个不同的表达蛋白，与对照组相比，NRMPP组、RMPP组分别鉴定出134（70个上调、64个下调）、126（63个上调、63个下调）个不同的表达蛋白。对鉴定出的蛋白进行综合功能分类和蛋白互作网络的分析揭示了RMPP复杂的发病机制。本研究首次构建了顽固性肺炎支原体肺炎相关蛋白的综合蛋白组图，为深入研究顽固性肺炎支原体肺炎的病理机制提供了基础。
 
外文摘要：Mycoplasma pneumoniae (MP) is a leading cause of community-acquired pneumonia in children and young adults. Although MP pneumonia is usually benign and self-limited, in some cases it can develop into life-threating refractory MP pneumonia (RMPP). However, the pathogenesis of RMPP is poorly understood. The identification and characterization of proteins related to RMPP could provide a proof of principle to facilitate appropriate diagnostic and therapeutic strategies for treating paients with MP. In this study, we used a quantitative proteomic technique (iTRAQ) to analyze MP-related proteins in serum samples from 5 patients with RMPP, 5 patients with non-refractory MP pneumonia (NRMPP), and 5 healthy children. Functional classification, sub-cellular localization, and protein interaction network analysis were carried out based on protein annotation through evolutionary relationship (PANTHER) and Cytoscape analysis. A total of 260 differentially expressed proteins were identified in the RMPP and NRMPP groups. Compared to the control group, the NRMPP and RMPP groups showed 134 (70 up-regulated and 64 down-regulated) and 126 (63 up-regulated and 63 down-regulated) differentially expressed proteins, respectively. The complex functional classification and protein interaction network of the identified proteins reflected the complex pathogenesis of RMPP. Our study provides the first comprehensive proteome map of RMPP-related proteins from MP pneumonia. These profiles may be useful as part of a diagnostic panel, and the identified proteins provide new insights into the pathological mechanisms underlying RMPP.
外文关键词：MACROLIDE RESISTANCE; INFECTIONS; INTERLEUKIN-18; PATHOGENESIS; ACTIVATION; CYTOKINES; PROTEINS; CHILDREN; ADULTS; CELLS
作者：Yu, JL; Song, QF; Xie, ZW; Jiang, WH; Chen, JH; Fan, HF; Xie, YP; Lu, G
作者单位：广州医科大学
期刊名称：JAPANESE JOURNAL OF INFECTIOUS DISEASES
期刊影响因子：1.21
出版年份：2017
出版刊次：9
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