中文摘要：研究将VP2和VP5保护性抗原基因融合在一起，并连接到鸡IgY Fc基因，将携带这种融合基因的真核表达质粒导入毕赤酵母，从而表达出重组VP2-VP5-Fc蛋白。应用这种重组蛋白作为免疫抗原评价疫苗的免疫反应，并以毕赤酵母表达的重组VP2-Fc蛋白、VP2蛋白以及商业化的VPN2亚单位疫苗作为对照。试验同时以实验室先前发现的一种免疫刺激剂TPPPS作为佐剂检测其对前述抗原的免疫调节作用。研究结果表明，与商业化的VP2亚单位相比，毕赤酵母表达的重组VP2亚单位疫苗表现出更高的免疫原性；VP2-Fc蛋白的效果优于VP2蛋白，VP2-VP5-Fc亚单位抗原能够进一步提供机体的免疫反应。此外，TPPPS是VP2-VP5-Fc亚单位疫苗的一种较好的免疫增强剂。因此，重组VP2-VP5-Fc亚单位抗原与TPPPS佐剂结合能够提高传染性法氏囊病毒疫苗对抗传染性法氏囊病毒的作用。
外文摘要： Infectious bursal disease virus (IBDV) is a highly contagious pathogen that causes damage in lymphoid organs and remains a threat to the poultry industry worldwide. Currently, subunit vaccines based on VP2 antigen expressed in prokaryotic systems are widely used in clinical settings. However, the immunogenicity of VP2 vaccines is limited because of their inherent defect that the structure of the antigen expressed in Escherichia coli (E. coli) may be different from its natural conformation. In this study, we fused VP2 and VP5 protective antigen genes and linked the chicken IgY Fc gene onto it. The eukaryotic expression plasmid carrying the fusion gene was transformed into Pichia pastoris (P. pastoris) to express the recombinant VP2-VP5-Fc protein. The recombinant protein was used as immunogen for evaluating immune response, and the recombinant VP2-Fc and VP2 proteins expressed in P. pastoris and the commercial VP2 subunit vaccines were used as controls. Moreover, Taishan Pinus massoniana pollen polysaccharide (TPPPS), an immunomodulator found by our laboratory, was used as adjuvant to investigate its immune modulatory effects on immunogens. Chickens were divided into six groups and inoculated with VP2-VP5-Fc+TPPPS, VP2-VP5-Fc, VP2-Fc, VP2 vaccine, commercial VP2 subunit vaccine, and phosphate buffered saline (PBS). The recombinant VP2 subunit vaccine expressed in P. pastoris exhibited higher immunogenicity than the commercial VP2 subunit vaccine. The VP2-Fc protein showed a better effect than the VP2 protein, and the VP2-VP5-Fc subunit further improved the immune effects. In addition, TPPPS was proved to be a good immunopotentiator for the VP2-VP5-Fc subunit vaccine. Hence, the recombinant VP2-VP5-Fc subunit combined with TPPPS adjuvant exhibits potential as efficient IBDV vaccine to prevent infectious bursal disease.
外文关键词：IBDV； IgY Fc； Pichia pastoris expression； subunit vaccine； TPPPS
作者：Wang, HN; Shan, SF; Wang, SJ; Zhang, H; Ma, LL; Hu, LP; Huang, H; Wei, K; Zhu, RL
作者单位：山东农业大学
期刊名称：FRONTIERS IN MICROBIOLOGY
期刊影响因子：4.07
出版年份：2017
出版刊次：11
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