中文摘要：为研发一种广谱H5亚单位疫苗，研究构建了一种重组HA序列（rHA），该序列可以在杆状病毒-昆虫细胞系统的病毒样颗粒中表达，并评估了这种rHA VLPs 疫苗的效率。与商业化的Re6 或Re6-CVCVA5 疫苗相比，单剂量免疫rHA VLPs 或rHA-CVCVA5 能够诱导产生更高的血清凝集素抑制滴度和中和抗体滴度，产生更多的粘膜抗体、IFN-γ和IL-4细胞因子，细胞毒性T淋巴细胞反应升高。在抗H5亚型病毒不同分支干扰方面，rHA VLPs疫苗优于商业化的Re6疫苗。本研究结果表明，H5亚型一致性HA病毒样颗粒单剂量免疫能够为鸡只提供广谱的抗HPAI保护作用。
外文摘要： The H5 subtype highly pathogenic avian influenza (HPAI) virus is one of the greatest threats to global poultry industry. To develop broadly protective H5 subunit vaccine, a recombinant consensus HA sequence (rHA) was constructed and expressed in virus-like particles (rHA VLPs) in the baculovirus-insect cell system. The efficacy of the rHA VLPs vaccine with or without immunopotentiator (CVCVA5) was assessed in chickens. Compared to the commercial Re6 or Re6-CVCVA5 vaccines, single dose immunization of chickens with rHA VLPs or rHA-CVCVA5 vaccines induced higher levels of serum hemagglutinin inhibition titers and neutralization titers, mucosal antibodies, IFN-gamma and IL-4 cytokines in sera, and cytotoxic T lymphocyte responses. The rHA VLPs vaccine was superior to the commercial Re6 vaccine in conferring cross-protection against different clades of H5 subtype viruses. This study reports that H5 subtype consensus HA VLP single dose vaccination provides broad protection against HPAI virus in chickens.
外文关键词：H5 subtype influenza virus； vaccine； consensus； virus-like particles； broad protection
作者：Wu, PP; Lu, JH; Zhang, XH; Mei, M; Feng, L; Peng, DX; Hou, JB; Kang, SM; Liu, XF; Tang, YH
作者单位：江苏省农业科学院
期刊名称：FRONTIERS IN IMMUNOLOGY
期刊影响因子：6.43
出版年份：2017
出版刊次：11
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