中文摘要：研究制备了一种重组H7HA（rH7HA）亚单位疫苗的佐剂PELC/CpG。肌肉免疫BALB/c小鼠后，这种重组H7HA疫苗中的佐剂能够代替明矾佐剂诱导产生更高水平IgG、血清抑制凝集素、病毒中和抗体，能够诱导脾脏产生更多的H7HA特异性IFN-γ分泌性T细胞以及抗体分泌细胞，并提高机体抗活病毒的保护作用。研究结果表明，rH7HA蛋白与PELC/CpG佐剂联用能够诱导产生潜在的抗H7N9免疫作用，该结果对制备H7N9亚单元有积极作用。
 
外文摘要：Humans infected with H7N9 avian influenza viruses can result in severe pneumonia and acute respiratory syndrome with an approximately 40% mortality rate, and there is an urgent need to develop an effective vaccine to reduce its pandemic potential. In this study, we used a novel PELC/CpG adjuvant for recombinant H7HA (rH7HA) subunit vaccine development. After immunizing BALB/c mice intramuscularly, rH7HA proteins formulated in this adjuvant instead of an alum adjuvant elicited higher IgG, hemagglutination-inhibition, and virus neutralizing antibodies in sera; induced higher numbers of H7HA-specific IFN-y-secreting T cells and antibody secreting cells in spleen; and provided improved protection against live virus challenges. Our results indicate that rH7HA proteins formulated in PELC/CpG adjuvant can induce potent anti-H7N9 immunity that may provide useful information for H7N9 subunit vaccine development.
外文关键词：PELC/CpG； Adjuvant； H7N9； Subunit vaccine
作者：Chen, TH; Liu, YY; Jan, JT; Huang, MH; Spearman, M; Butler, M; Wu, SC
作者单位：国立清华大学（台湾）
期刊名称：ANTIVIRAL RESEARCH
期刊影响因子：4.5
出版年份：2017
出版刊次：10
点击下载：应用重组血凝素蛋白制备一种新型的H7N9亚单位疫苗的佐剂PELC/CpG