中文摘要：本研究利用不同数量的C3d作为分子佐剂（n = 1, 2, 4, or 6）构建了一个转移载体，并克隆到真核表达载体(pVAXI-optiF)上，以表达新城疫病毒的F基因，并分别命名为pVAXI-F(o)-C3d1、pVAXI -F(o)-C3d2、pVAXI-F(o)-C3d4和pVAXI-F(o)-C3d6。细胞转染试验表明，pVAXI-F(o)-C3d6表达量最高。体外免疫试验表明，以pVAXI-F(o)-C3d6皮下免疫鸡群与其他载体相比能够诱导产生更好的免疫反应。pVAXI-F(o)-C3d6对新城疫病毒F48E9的免疫保护效率为80%。这些结果表明，C3d6能够作为一种分子佐剂快速诱导产生有效的抗新城疫病毒的免疫反应。
 
外文摘要：Newcastle disease is a serious infectious disease in the poultry industry. The commercial vaccines can only offer limited protection and some of them are expensive and need adjuvants. At present, DNA vaccines are widely used. However, the immune responses induced by DNA vaccines are too slow and low. Here, we constructed the transfer vectors with a different number of C3d as molecular adjuvants (n = 1, 2, 4, or 6), and the vectors were cloned into the optimal eukaryotic expression plasmid (pVAXI-optiF) that expressed the F gene of Newcastle disease virus (NDV), and named pVAXI-F(o)-C3d1, pVAXI -F(o)-C3d2, pVAXI-F(o)-C3d4, and pVAXI-F(o)-C3d6, respectively. Cell transfection test indicated that pVAXI-F(o)-C3d6 showed the highest expression. In vivo immunization showed that the chickens immunized with pVAXI-F(o)-C3d6 intramuscularly induced better immune responses than the chickens immunized with the other plasmids. The protective efficacy of pVAXI-F(o)-C3d6 was 80% after challenge with the highly virulent NDV strain F48E9. The results in this study showed that C3d6 could be used as a molecular adjuvant to quickly induce an effective immune response to control NDV.
外文关键词：Newcastle disease virus, DNA vaccine, F gene, C3d, molecular adjuvant
作者： Zhao, K; Duan, XT; Hao, LW; Wang, XH; Wang, YF
作者单位：黑龙江大学
期刊名称：JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
期刊影响因子：1.6
出版年份：2017
出版刊次：11
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