中文摘要：研究开发了一种脂质体狂犬病疫苗（LipoRV），该疫苗能够激活BALB/c小鼠产生狂犬病毒中和抗体。脂质体溶液由氢化大豆磷脂和胆固醇组成，LipoRV由脂质体溶液和灭活狂犬病毒疫苗（IRV）组成。试验结果表明，与单独使用灭活疫苗IRV相比，LipoRV免疫组IL-2、IFN-浓度更高，自杀性细胞活性更好；LipoRV的效能优于IRV。此外，采用LipoRV免疫3次后小鼠的存活率高于IRV免疫5次的存活率。该结果表明，脂质体能够增强小鼠对狂犬病疫苗的免疫反应，可作为一种免疫促进剂进行应用。
 
外文摘要：Essen regimen, the Thai Red Cross two-site ID regimen, Zagreb schedule, and the eight-site ID regimen are the standard rabies vaccines recommended by the World Health Organization (WHO). In this study, a liposomal rabies vaccine (LipoRV) was developed, which was found to facilitate the production of rabies virus neutralizing antibody (RVNA) in BALB/c mice. Liposome solution was prepared with hydrogenated soya phosphatide and cholesterol. LipoRV composed of liposome solution and inactivated rabies vaccine (IRV). The immune responses were compared between the mice treated with either LipoRV or IRV. Higher levels of interleukin-2 (p < 0.05), interferon- (p < 0.01), and natural killer cell activity (p < 0.05) were observed in the mice immunized with LipoRV than those with IRV. The potency of LipoRV was significantly higher than that IRV (p < 0.05). In addition, three injections of LipoRV on days 0, 3, and 14 could elicit similar RVNA levels as the five shots of IRV. Our data also showed a higher survival rate in mice treated with three shots of LipoRV (56.2%) than five shots of IRV (40.6%). In conclusion, liposome enhances the immune response of mice to rabies vaccine and could be applied as a potential immunopotentiator.
外文关键词： liposomal rabies vaccine, liposome, immune response, rabies vaccine
作者：Miao, L; Yang, Y; Yan, MK; Li, YC; Zhao, JS; Guo, JS; Zheng, D
作者单位：吉林大学
期刊名称：VIRAL IMMUNOLOGY
期刊影响因子：1.26
出版年份：2017
出版刊次：12
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