中文摘要：研究构建了三种PED亚单位疫苗，这些疫苗采用重组沙门氏菌鞭毛（rSF）作为粘膜分子佐剂。将重组沙门氏菌鞭毛（rSF）中D3区域用猪流行性腹泻病毒（PEDV）中的COE区域代替，形成rSF-COE-3D。COE融合到rSF C'/N' 末端构建rSF-COE-C和rSF-COE-N。结果表明，rSF-COE-3D能够显著提高COE特异性抗体浓度，包括血浆IgG\IgA、粘膜IgA和PEDV中和抗体浓度；rSF-COE-3D能够诱导小鼠脾细胞产生更多的CD3(+)CD8*T细胞、提高IFN-g和IL-4的浓度。总而言之，该研究表明，rSF-COE-3D能够提高小鼠特异性体液免疫和粘膜免疫反应，表明rSF-COE-3D可作为一种有效的新型母源PED疫苗进行推广。
外文摘要：Porcine epidemic diarrhea (PED) is an important re-emergent infectious disease and inflicts huge economic losses to the swine industry worldwide. To meet the pressing need of developing a safe and cost-efficient PED maternal vaccine, we generated three PED subunit vaccine candidates, using recombined Salmonella flagellin (rSF) as a mucosal molecular adjuvant. Domain D3 in rSF was replaced with COE domain of PEDV to generate rSF-COE-3D. COE fused to the flanking C'/N' terminal of rSF yielded rSF-COE-C and rSF-COE-N. As a result, rSF-COE-3D could significantly improve COE specific antibody production including serum IgG, serum IgA, mucosal IgA and PEDV neutralizing antibody. Furthermore, rSF-COE-3D elicited more CD3(+)CD8*T cell and cytokine production of IFN-gamma and IL-4 in mouse splenocytes. In summary, our data showed that rSF-COE-3D could improve specific humoral and mucosal immunity in mice, thus suggesting that rSF-COE-3D could be applied as a novel efficient maternal PED vaccine.
外文关键词：Porcine epidemic diarrhea, COE, Flagellin, Subunit vaccine
作者：Li, Qianniu; Peng, Ouyang; Wu, Tingting; Xu, Zhichao; Huang, Licheng
作者单位：中山大学
期刊名称：VACCINE
期刊影响因子：3.235
出版年份：2018
出版刊次：3
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