中文摘要：本研究采用阳离子聚合物GenJet (TM)或外源-jetPEI (R)构建了一种H5N1流感疫苗，并通过小鼠模型试验表明加入GenJet (TM)或外源-jetPEI (R)能够显著增强H5N1疫苗的免疫原性，产生免疫性保护作用。对适应性免疫应答详细分析表明，采用GenJet (TM)或外源-jetPEI (R)构建的H5N1流感疫苗均能诱导混合性T(H)1/T(H)2抗原特异性CD4 T细胞反应、抗原特异性细胞性CD8 T细胞核记忆B细胞反应。该研究结果表明，阳离子聚合物可作为疫苗佐剂用作免疫原性较弱疫苗的粘膜传递剂。
外文摘要：Avian influenza virus infection is a serious public health threat and preventive vaccination is the most cost-effective public health intervention strategy. Unfortunately, currently available unadjuvanted avian influenza vaccines are poorly immunogenic and alternative vaccine formulations and delivery strategies are in urgent need to reduce the high risk of avian influenza pandemics. Cationic polymers have been widely used as vectors for gene delivery in vitro and in vivo. In this study, we formulated H5N1 influenza vaccines with GenJet (TM) or in vivo-jetPEI (R), and showed that these formulations significantly enhanced the immunogenicity of H5N1 vaccines and conferred protective immunity in a mouse model. Detailed analyses of adaptive immune responses revealed that both formulations induced mixed T(H)1/T(H)2 antigen-specific CD4 T-cell responses, antigen-specific cytotoxic CD8 T-cell and memory B-cell responses. Our findings suggest that cationic polymers merit future development as potential adjuvants for mucosal delivery of poorly immunogenic vaccines.
外文关键词：Cationic polymers, H5N1 vaccine, adaptive immunity, protective immunity, mouse model, influenza
作者：Cao, WP; Mishina, M; Amoah, S; Mboko, WP; Bohannon, C; Mccoy, J; Mittal, SK; Gangappa, S; Sambhara, S
作者单位：美国疾病预防与控制中心
期刊名称：DRUG DELIVERY
期刊影响因子：6.4
出版年份：2018
出版刊次：3
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