中文摘要：已有的研究表明，PCEP在猪和小鼠的多种疫苗中均表现出了佐剂特性。本研究将灭活猪流感（SIV）H1N1疫苗与PCEP联合，通过肌肉或常规皮内免疫猪只，分析猪只产生的免疫反应类型和程度。结果表明，皮内注射PCEP为佐剂的灭活Sly疫苗能够显著刺激抗SIV抗体的滴度，提高中和抗体浓度，显著降低猪只肺部病毒载荷量和肺部病变程度。这些结果表明，PCEP作为疫苗佐剂能有效对抗猪群的流感病毒，是一种前景广阔的猪流感疫苗皮内免疫佐剂。
 
外文摘要：Swine influenza virus is endemic worldwide and it is responsible for significant economic losses to the swine industry. A vaccine that stimulates a rapid and long-lasting protective immune response to prevent this infection is highly sought. Poly[di(sodium carboxylatoethylphenoxy)-phosphazene (PCEP) has demonstrated adjuvant activity when formulated as part of multiple vaccines in mice and pigs. In this study we examined the magnitude and type of immune response induced in pigs vaccinated via the intramuscular or intradermal routes with inactivated swine influenza virus (SIV) H1N1 vaccine formulated with PCEP. Intradermal administration of PCEP-adjuvanted inactivated Sly vaccine stimulated significant anti-SIV antibody titres, increased neutralizing antibodies, and significantly reduced lung virus load with limited reduction of gross lung lesions after challenge with virulent H1N1 relative to control animals. These results indicate that PCEP may be effective as a vaccine adjuvant against swine influenza viruses in pigs and should be considered a potential candidate adjuvant for future swine intradermal influenza vaccines. (C) 2018 Elsevier Ltd. All rights reserved.
外文关键词：Vaccine, Swine, Influenza, Route, Neutralizing antibodies, Protection
作者：Magiri, R; Lai, K; Chaffey, A; Zhou, Y; Pyo, HM; Gerdts, V; Wilson, HL; Mutwiri, G
作者单位：加拿大萨斯喀彻温大学
期刊名称：VACCINE
期刊影响因子：3.235
出版年份：2018
出版刊次：3
点击下载：灭活猪流感疫苗与佐剂PCEP联合进行皮内免疫能够诱导猪产生体液免疫和细胞免疫，并降低肺部病毒滴度