中文摘要：研究以BALB/c小鼠为模型通过皮下注射分析了CpG作为HIV-1-tat DNA疫苗佐剂的效果。试验将HIV-1-tat基因克隆到pcDNA3.1载体上，分析皮下免疫后小鼠的体液免疫和细胞免疫反应。结果表明：注射HIV-1-tat后，小鼠IFN-γ细胞因子的分泌水平、淋巴细胞增殖和IgG水平显著提高，这些指征在最后一次注射后的2周后出现。将HIV-1-tat DNA疫苗与CpG-ODNs佐剂同时皮下注射能够诱导产生有效的抗HIV-1感染的细胞免疫反应。
外文摘要：To evaluate the combined effects of CpG oligodeoxynucleotides (CpG-ODNs) adjuvant and subcutaneous injection route on efficacy of a HIV-1-tat DNA vaccine candidate using BALB/c mice as an animal model. Evaluation of cellular and humoral immunity of mice injected subcutaneously with HIV-1-tat gene cloned into a pcDNA3.1 vector indicated that significant levels of IFN-gamma cytokine secretion (900 pg/ml), lymphocyte proliferation (2.5 stimulation index) and IgG(2a) (1.45 absorbance 450 nm) production could be achieved. These indicators of stimulated cellular immunity were elicited 2 weeks after the last injection (P < 0.05). Formulation of HIV-1-tat DNA vaccine candidate with CpG-ODNs as an adjuvant while administrated subcutaneously are a promising approach to induce effective cellular immunity responses against HIV-1 infection.
外文关键词：CpG-ODNs, DNA vaccine, HIV-1, Subcutaneous injection, Tat, HIV vaccine
作者：Panahi, Z; Abdoli, A; Mosayebi, G; Mahdavi, M; Bahrami, F
作者单位：伊朗 Arak Univ Med Sci
期刊名称：BIOTECHNOLOGY LETTERS
期刊影响因子：1.73
出版年份：2018
出版刊次：3
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