中文摘要：本研究将重组乙型肝炎抗原（rHBsAg）负载到甘露醇糖基化壳聚糖（MC）纳米颗粒上，构建了靶向疫苗传递载体。通过离子交换法合成纳米颗粒，并分析了其物化特性、细胞毒性和抗原性。rHBsAg-MC纳米颗粒的体外释放速率为，最初7天可释放26%，此后进入缓释期，49天中释放了25%，该释放动力学与Higuchi 模型相似。rHBsAg-MC纳米颗粒的细胞毒性具有时间和剂量效应。该结果表明，负载rHBsAg的纳米颗粒是促进靶向抗原进入机体免疫系统的一种有效途径。
 
外文摘要：The design of effective vaccine delivery system is opening up new feasibilities for making immunization more safe and efficient. In this study, recombinant hepatitis B virus surface antigen (rHBsAg) was loaded in mannosylated chitosan (MC) nanoparticles to be used as a targeted vaccine delivery vehicles. The nanoparticles were prepared by ionic gelation method and characterized for physicochemical properties, cytotoxicity and antigenicity. The rHBsAg-loaded MC nanoparticles showed spherical shape with mean particle size of 246 +/- 33 nm, zeta potential of 25.6 +/- 1.7 mV, loading capacity of 12.2 +/- 1.4%, and encapsulation efficiency of 90 +/- 1.6%. In vitro release profile of rHBsAg-loaded MC nanoparticles exhibited an initial burst release of about 26% in the first 7 days followed by a slow release of 25% for 49 days, with release kinetic similar to Higuchi model. SDS-PAGE analysis confirmed integrity of released rHBsAg and structural stability of the antigen during entrapment process. The rHBsAg-loaded MC nanoparticles indicated time-and concentration-dependent cytotoxicity using MTT assay. It can be concluded that entrapment of rHBsAg in MC nanoparticles appears to be a suitable approach for targeting this antigen into body immune system.
外文关键词：Mannosylated chitosan nanoparticles, rHBsAg, Cytotoxicity, Immune system
作者：Mehrabi, M; Dounighi, NM; Rezayat, SM; Doroud, D; Amani, A; Khoobi, M; Ajdary, S
作者单位：伊朗德黑兰医科大学
期刊名称：JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
期刊影响因子：1.25
出版年份：2018
出版刊次：4
点击下载：一种提高疫苗免疫原性的新方法——利用负载重组乙型肝炎抗原的甘露醇糖基化壳聚糖纳米颗粒作为靶向疫苗的传递系统