中文摘要：研究合成了两种层状双氢氧化物纳米复合物，即纳米粒子（NPs）和纳米片（NSs），分析了这两种复合物对促进抗肿瘤免疫反应的作用。结果发现，两种纳米复合物传递的卵黄免疫原（OVA）与免疫促进剂（TLR9配体CpG）联合使用均能提高机体的系统和细胞免疫反应，表现为IgG1、IgG2a和IFN-γ水平的升高。试验比较发现，层状双氢氧化物纳米片促进特异性抗体反应的活性高于层状双氢氧化物纳米粒子，但两者诱导的细胞免疫反应相似。采用OVA-CpG疫苗联合两种纳米复合物免疫小鼠发现，免疫后癌细胞的生长受到抑制，小鼠存活期更长。综上，这些研究表明层状双氢氧化物纳米粒子和纳米片可作为一种纳米佐剂提高蛋白为基础的抗肿瘤疫苗的效率。
外文摘要：Effective adjuvants in anti-tumour vaccine formulations are very important in the development of newgeneration vaccines. In this study, two layered double hydroxide (LDH) nanomaterial forms, i.e. nanoparticles (NPs) and nanosheets (NSs), were synthesised and examined as adjuvants to provoke the immune responses for anti-tumour purpose. Immunogen ovalbumin (OVA) delivered by both nanomaterials induced much stronger humoral and cell-medicated immune responses, together with an immune stimulant (TLR9 ligand CpG), as evidenced by higher levels of IgG1, IgG2a and interferon-gamma. By comparison, LDH NSs showed higher activity to promote specific antibody responses than LDH NPs but with a similar cell-mediated immune response. The mice immunised with OVA-CpG vaccines formulated with both nanomaterials showed stronger inhibition of the inoculated tumour growth and had a longer survival. Altogether, these data indicate that LDH NPs and NSs can be used as potential nanoadjuvants for efficient protein-based anti-tumour vaccines.
外文关键词：TOLL-LIKE RECEPTORS; DENDRITIC CELLS; COLLOIDAL STABILITY; CROSS-PRESENTATION; CANCER VACCINES; CPG OLIGONUCLEOTIDES; IMMUNE ADJUVANTS; CLASS-I; ANTIGEN; DELIVERY
作者：Yan, SY; Gu, WY; Zhang, B; Rolfe, BE; Xu, ZP
作者单位：澳大利亚昆士兰大学
期刊名称：DALTON TRANSACTIONS
期刊影响因子：3.85
出版年份：2018
出版刊次：3
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