中文摘要：研究制备了3种纳米乳剂佐剂（封装、混合液和联合体），利用耐甲氧西林金葡菌感染模型探索了这3种佐剂的免疫增强作用及其作用机制。结果表明，耐甲氧西林金葡菌抗原的3种不同融合方式均能提供系统免疫和细胞免疫反应；与混合、联合体相比，抗原纳米乳剂封装组更能促进抗原呈递细胞（DCs）摄取抗原，引流淋巴结中DCs的活性更高且注射部位的缓释作用更好。同时，封装组诱导机体产生抗耐甲氧西林金葡菌保护作用的效率更高，表现为更高的抗体反应和Th1/Th17偏向CD4(+) T细胞反应的增强。该研究结果表明，采用纳米乳剂封装抗原的方式附着疫苗是一种促进机体形成抗耐甲氧西林金葡菌感染的保护作用的有效途径。
 
外文摘要：Nanoemulsion adjuvants-based vaccines have potent induced immune responses against methicillin-resistant Staphylococcus aureus (MRSA) infection. However, the efficacies and immune responses of different antigen-attaching ways on self-made nanoemulsion adjuvants remain unknown. In this study, we designed three formulations of nanoemulsion adjuvants (encapsulation, mixture, and combination) to explore their immune response-enhancing effects and their underlying mechanism in a systemic infection model of MRSA. Our results showed that the three nanoemulsion-attachment ways formulated with a fusion antigen of MRSA (Hla(H35L)IsdB(348-465)) all improved humoral and cellular immune responses. When compared with the mixture and combination formulations, the nanoemulsion-encapsulation group effectively promoted the antigen uptake of dendritic cells (DCs) in vitro, the activation of DC in draining lymph nodes and the delayed release of antigen at injection sites in vivo. Moreover, the encapsulation group induced a more ideal protective efficacy in a MRSA sepsis model by inducing more potent antibody responses and a Th1/Th17 biased CD4(+) T cell response when compared with the other two attachment ways. Our findings suggested that the encapsulated formulation of vaccine with nanoemulsion adjuvant is an effective attachment way to provide protective immunity against MRSA infection.
外文关键词： DELIVERY-SYSTEM; NANOPARTICLE VACCINES; DRUG-DELIVERY; T-CELLS; ALUM; IMMUNOGENICITY; DISEASES; PROMOTE; CANCER; SIZE
作者：Yang, LY; Wei, C; Yang, Y; Tong, YN; Yang, S; Peng, LS; Zuo, QF; Zhuang, Y; Cheng, P; Zeng, H; Zou, QM; Sun, HW
作者单位：中国人民解放军第三军医大学
期刊名称：RSC ADVANCES
期刊影响因子：3.06
出版年份：2018
出版刊次：4
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