中文摘要：研究以小鼠为对象，分析了大豆油中人参根皂苷（SO-GS-R）对口蹄疫疫苗的佐剂作用。当SO-GS-R与口蹄疫疫苗联合免疫小鼠后，小鼠血浆抗体水平和细胞因子反应显著高于单独使用口蹄疫抗原免疫的小鼠。对血浆和肌肉组织中细胞因子浓度的测定结果表明，SO-GS-R能够募集中性白细胞、嗜酸性细胞、T细胞和巨噬细胞，从而在免疫部位引起免疫细胞的募集，激发抗原呈递细胞参与免疫反应，增强免疫反应。
外文摘要：In the present study, the adjuvant effect of soybean oil containing ginseng root saponins (SO-GS-R) on the immune response to foot-and-mouth disease vaccine (FMDV) in mice was investigated. When immunized with FMDV antigen emulsified in an SO-GS-R formulation, mice generated remarkably higher serum antibody and cytokine responses than mice immunized with FMDV antigen alone. To elucidate the mechanisms underlying the adjuvant effect of SO-GS-R, we measured cytokines in serum and muscle tissue after intramuscular injection of SO-GS-R. The results showed that injection of SO-GS-R significantly increased the levels of IL-1, IL-5, IL-6, G-CSF, KC, MCP-1, MIP-1, and MIP-1 in both serum and muscle. These results suggested that SO-GS-R recruits neutrophils, eosinophils, T cells and macrophages, causing immune cell recruitment at the injection site, driving antigen-presenting cells to actively participate in the onset of immunity, and amplifying the immune responses. Considering its adjuvant activity and plant-derived properties, SO-GS-R should be further studied for its adjuvant effect on vaccines used in food animals.
外文关键词： adjuvant, cytokine, ginseng saponin, soybean oil
作者：CR; Xu, W; Chen, J; Guan, R; Bi, SC; Jin, HB; Cui, XM; Shi, FS; Hu, SH
作者单位：浙江大学
期刊名称：MICROBIOLOGY AND IMMUNOLOGY
期刊影响因子：1.57
出版年份：2018
出版刊次：3
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