中文摘要：外分泌体通过转运功能性蛋白和RNAs参与调节细胞交互。本研究鉴定出一种肺源外分泌体微小RNA(miRNAs)，分析外分泌体在流感病毒感染过程中的作用。当采用不同流感病毒感染小鼠时，在所有检测到的miRNAs中，支气管肺泡灌洗液中miR-483-3p的浓度较高。将miR-483-3p转染MLE-12细胞，当MLE-12细胞感染病毒时，miR-483-3p能够激发I型干扰素和促炎性细胞因子的基因表达。研究发现，RNF5是miR-483-3p的靶向基因；并且miR-483-3p的另一个靶向细胞基因CD81，是MLE-12细胞RIG-I信号通路中的反向调控因子。总而言之，该研究表明，BALF外分泌体miRNAs能够调控机体对抗病毒感染的抗病毒反应和炎性反应。
 
外文摘要：Exosomes regulate cell-cell communication by transferring functional proteins and RNAs between cells. Here, to clarify the function of exosomes during influenza virus infection, we characterized lung-derived exosomal micro RNAs (miRNAs). Among the detected miRNAs, miR-483-3p was present at high levels in bronchoalveolar lavage fluid (BALF) exosomes during infection of mice with various strains of influenza virus, and miR-483-3p transfection potentiated gene expression of type I interferon and proinflammatory cytokine upon viral infection of MLE-12 cells. RNF5, a regulator of the RIG-I signaling pathway, was identified as a target gene of miR-483-3p. Moreover, we found that CD81, another miR-483-3p target, functions as a negative regulator of RIG-I signaling in MLE-12 cells. Taken together, this study indicates that BALF exosomal miRNAs may mediate the antiviral and inflammatory response to influenza virus infection.
外文关键词：influenza virus, exosome, microRNA, innate immunity
作者：Maemura, T; Fukuyama, S; Sugita, Y; Lopes, TJS; Nakao, T; Noda, T; Kawaoka, Y
作者单位：日本东京大学
期刊名称：JOURNAL OF INFECTIOUS DISEASES
期刊影响因子：6.273
出版年份：2018
出版刊次：3
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