中文摘要：小肠粘膜免疫损伤会显著增加急性和慢性疾病的发病风险，IgA通过提供抗病原和毒素的保护作用在系统粘膜免疫中起到重要作用。研究分析了一种串联重复-β-半乳糖苷绑定蛋白——内源性半乳凝素-9对小肠粘膜免疫的作用。试验发现，缺乏半乳凝素-9会导致内脏中粘膜抗原特异性IgA反应丧失，半乳凝素-9对适应性IgA反应至关重要，且Th17-IgA轴参与了整个过程。该研究表明，半乳凝素-9通过Th17-IgA轴在粘膜适应性免疫反应过程中起重要作用，调控半乳凝素-9的表达或活性能够改变小肠粘膜免疫反应，这一发现对开发粘膜疫苗具有重要意义。
外文摘要：Impairment of the intestinal mucosal immunity significantly increases the risk of acute and chronic diseases. IgA plays a major role in humoral mucosal immunity to provide protection against pathogens and toxins in the gut. Here, we investigated the role of endogenous galectin-9, a tandem repeat-type beta-galactoside -binding protein, in intestinal mucosal immunity. By mucosal immunization of Lgals9(-/-) and littermate control mice, it was found that lack of galectin-9 impaired mucosal antigen-specific IgA response in the gut. Moreover, Lgals9(-/-) mice were more susceptible to developing watery diarrhea and more prone to death in response to high-dose cholera toxin. The results indicate the importance of galectin-9 in modulating intestinal adaptive immunity. Furthermore, bone marrow chimera mice were established, and galectin-9 in hematopoietic cells was found to be critical for adaptive IgA response. In addition, immunized Lgals9(-/-) mice exhibited lower expression of Il17 and fewer T helper 17 (Th17) cells in the lamina propria, implying that the Th17-IgA axis is involved in this mechanism. Taken together, these findings suggest that galectin-9 plays a role in mucosal adaptive immunity through the Th17-IgA axis. By manipulating the expression or activity of galectin-9, intestinal mucosal immune response can be altered and may benefit the development of mucosal vaccination.
外文关键词：IGA RESPONSES; INTESTINAL IGA; DOWN-REGULATION; PEYERS-PATCHES; LAMINA PROPRIA; T(H)17 CELLS; PLASMA-CELLS; TH17 CELLS; GUT; HOMEOSTASIS
作者：Liang, CC; Li, CS; Weng, IC; Chen, HY; Lu, HH; Huang, CC; Liu, FT
作者单位：中国台湾中央研究院
期刊名称：AMERICAN JOURNAL OF PATHOLOGY
期刊影响因子：4.057
出版年份：2018
出版刊次：5
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