中文摘要：研究利用多糖可以促进Th1和Th2反应的优点，构建了一种磷酸盐凝胶多糖-O-交联季铵化壳聚糖纳米颗粒（CS/O-HTCC），并分析了这种纳米颗粒的佐剂特性。结果表明，基于多糖的纳米颗粒能够诱导抗原呈递细胞的增殖与激活；以卵清蛋白（Ova）抗原为试验表明，抗原蛋白负载效率高，且抗原与佐剂复合物（CS/O-HTCC）较易为上皮细胞摄取；研究证实，Ova/ CS/O-HTCC纳米颗粒诱导了抗原呈递细胞的激活和成熟，显著促进了淋巴细胞增殖和分化，显著提高了Ova特异性抗体浓度。因此，CS/O-HTCC纳米颗粒是一种理想的疫苗佐剂，适用于可溶性抗原的鼻内或粘膜免疫。
外文摘要：Introduction: The development of ideal vaccine adjuvants for intranasal vaccination can provide convenience for many vaccinations. As an ideal intranasal vaccine adjuvant, it should have the properties of assisting soluble antigens to pass the mucosal barrier and potentiating both systemic and mucosal immunity via nasal administration. Methods: By using the advantages of polysaccharides, which can promote both T-helper 1 and 2 responses, curdlan sulfate (CS)-O-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (O-HTCC) nanoparticles were prepared by interacting CS with O-HTCC, and the adjuvancy of the nanoparticles was investigated. Results: The results showed that the polysaccharide-based nanoparticles induced the proliferation and activation of antigen-presenting cells. High protein-loading efficiency was obtained by testing with the model antigen ovalbumin (Ova), and the Ova adsorbed onto the cationic CS/O-HTCC complexes was taken up easily by the epithelium. To evaluate the capacity of the Ova/CS/O-HTCC nanoparticles for immune enhancement in vivo, we collected and analyzed immunocytes, serum, and mucosal lavage fluid from intranasally vaccinated mice. The results showed that Ova/CS/O-HTCC nanoparticles induced activation and maturation of antigen-presenting cells and provoked the proliferation and differentiation of lymphocytes more significantly compared to the immunization of Ova mixed with aluminum hydroxide gel. Furthermore, CS/O-HTCC evoked a significantly higher level of Ova-specific antibodies. Conclusion: Therefore, these results suggest that CS/O-HTCC nanoparticles are ideal vaccine adjuvants for soluble antigens used in intranasal or mucosal vaccination.
外文关键词：curdlan sulfate, O-linked quaternized chitosan, nasal mucosa immunization, nanoparticle, adjuvant, ovalbumin, immune response
作者：Zhang, S; Huang, SS; Lu, L; Song, XL; Li, PL; Wang, FS
作者单位：山东大学
期刊名称：INTERNATIONAL JOURNAL OF NANOMEDICINE
期刊影响因子：4.3
出版年份：2018
出版刊次：4
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