2个相近猪群体中肺炎支原体遗传多样性分析

Persistence of the same genetic type of Mycoplasma hyopneumoniae in a closed herd for at least two years

点击次数:432   下载次数:276
中文摘要:研究于2013年-2015年对2个猪场中存在的肺炎支原体进行了监控,采用多位点可变数目串联重复序列分析法(MLVA)针对P97 R1、P146 R3 和 H4 位点进行了临床标本的肺炎支原体多样性分析。结果发现,2015年8月采集样本中的MLVA的多态性与2013年7月的样本一致,因此在两个相近的猪群中,同一遗传类型的肺炎支原体至少可以保持2年的时间。另外,对于非侵入性临床标本应用巢式PCR进行MLVA分型是可行的。
 
外文摘要:Two cross-sectional studies were carried out in 2013 and 2015 monitoring for Mycoplasma hyopneumoniae presence in a swine farm. In these studies, the genetic diversity of M. hyopneumoniae was assessed in clinical specimens using a Multiple Locus Variable-number tandem repeat Analysis (MLVA) targeting P97 R1, P146 R3 and H4 loci. The samples from August 2015 showed the MLVA profile prevalent in June 2013, therefore it can be concluded that a same genetic type of M. hyopneumoniae can persist for at least two years in a closed herd. In addition, the nested PCR reactions implemented in this study showed to be useful for MLVA typing in non-invasive clinical samples.
外文关键词:Swine, Mycoplasma hyopneumoniae, Typing, Multiple Locus Variable-number tandem repeat Analysis, Persistence, Polymerase chain reaction
作者:Rebaquea, F; Camacho, P; Parada, J; Lucchesi, P; Ambrogi, A; Tamiozzo, P
作者单位:加州大学里约热内卢大学
期刊名称:REVISTA ARGENTINA DE MICROBIOLOGIA
期刊影响因子:0.51
出版年份:2018
出版刊次:6
点击下载:2个相近猪群体中肺炎支原体遗传多样性分析
  1. 编译服务:动物支原体学
  2. 编译者:程金花
  3. 编译时间:2018-06-26