中文摘要：肺炎支原体肺炎是最普遍的社区获得性肺炎之一。本研究旨在探索儿童感染肺炎支原体肺炎后病原免疫反应。研究收集了6名支原体肺炎患儿的支气管肺泡灌洗液，并以3名异物吸入儿童的支气管肺泡灌洗液为对照，采用下一代转录组测序对样本进行了分析，并以荧光定量PCR进行验证。结果表明，与对照组相比，肺炎支原体肺炎组支气管肺泡灌洗液中发现了810个差异表达基因，其中412个基因包括RLTPR、CARD1 1和RASAL3表达上调，这些表达上调的基因集中于单核细胞增殖和生物信号传导过程中。京都基因和基因组分析表明，肺炎支原体肺炎组儿童造血细胞生成路径、自杀性T细胞调控细胞毒性路径和T细胞受体信号路径得到了显著上调。另外，GNLY 和 SLC11A1基因出现了显著的可变剪接现象，这有可能导致基因表达的变化。该研究揭示了肺炎支原体肺炎患儿体内NK和CD8+ T细胞的过激活和炎性化现象，IFNy、PRF1、GZMB、FASL和GNLY基因的上调可能在病原的感染过程中起重要作用。
外文摘要：Background: Mycoplasma pneumoniae pneumonia (MPP) is one of the most common community-acquired pneumonia; this study is to explore the immune-pathogenesis of children MPP. Methods: Next-generation transcriptome sequencing was performed on the bronchoalveolar lavage fluid cells from six children with MPP and three children with foreign body aspiration as control. Some of the results had been validated by quantitative real-time PCR in an expanded group of children. Results: Results revealed 810 differentially expressed genes in MPP group comparing to control group, of which 412 genes including RLTPR, CARD1 1 and RASAL3 were upregulated. These upregulated genes were mainly enriched in mononuclear cell proliferation and signaling biological processes. Kyoto encyclopedia of genes and genomes pathway analysis revealed that hematopoietic cell linage pathway, natural killer cell-mediated cytotoxicity pathway, and T cell receptor signaling pathway were significantly upregulated in MPP children. In addition, significant alternative splicing events were found in GNLY and SLC11A1 genes, which may cause the differential expressions of these genes. Conclusion: Our results suggest that NK and CD8+ T cells are over activated and proliferated in MPP children; the upregulated IFNy, PRF1 , GZMB, FASL, and GNLY may play important roles in the pathogenesis of children MPP.
外文关键词：Mycoplasma pneumoniae pneumonia； bronchoalveolar lavage fluid； children； natural killer cells； CD8+T cells； interferon gamma
作者：Gao, M; Wang, K; Yang, MY; Meng, FZ; Lu, RH; Zhuang, HD; Cheng, GH; Wang, XS
作者单位：吉林大学
期刊名称：FRONTIERS IN IMMUNOLOGY
期刊影响因子：6.429
出版年份：2018
出版刊次：6
点击下载：对肺炎支原体肺炎儿童支气管肺泡灌洗液转录分析揭示出自杀性和T细胞炎性反应