中文摘要：真菌毒素脱氧雪腐镰刀菌烯醇（DON）是谷物中最常见的污染物之一。猪对DON高度敏感，常因采食被DON污染的谷物而产生一系列的免疫反应，并有可能影响到一些特殊的细菌性疾病病原的感染。因此，欧盟委员会规定猪对DON的最大耐受值为900 mu g/kg日粮。本研究旨在探索日粮中中高浓度（1514 mu g/kg）的DON对感染肺炎支原体的断奶仔猪的影响，主要观察指标包括肺部病变、感染8-35天之间呼吸系统疾病评分（RDS），试验分为阴性对照组（NCG）、DON污染组（DON）、DON污染和肺炎支原体感染组（DONMHYO）和肺炎支原体感染组（MHYO）。结果表明：DONMHYO和MHYO组肺部系统病变、RDS、肺病理组织病变和肺炎支原体DNA拷贝数没有显著差异；与MHYO组相比，DONMHYO组肺炎支原体的感染程度并未加深。本研究结果表明，在本试验条件下断奶仔猪日粮中中高水平的DON污染并不会增加肺炎支原体肺炎的感染程度；在多重毒性、多重病原环境下DON对猪肺炎支原体感染的影响如何还有待进一步的试验验证。
 
外文摘要：Background: The mycotoxin deoxynivalenol (DON) is highly prevalent in cereals in moderate climates and therefore pigs are often exposed to a DON-contaminated diet. Pigs are highly susceptible to DON and intake of DON-contaminated feed may lead to an altered immune response and may influence the pathogenesis of specific bacterial diseases. Therefore, the maximum guidance level in feed is lowest in this species and has been set at 900 mu g/kg feed by the European Commission. This study aimed to determine the effect of in-feed administration of a moderately high DON concentration (1514 mu g/kg) on the severity of an experimental Mycoplasma hyopneumoniae (M. hyopneumoniae) infection in weaned piglets. Fifty M. hyopneumoniae-free piglets were assigned at 30 days of age [study day (D)0] to four different groups: 1) negative control group (NCG; n = 5), 2) DON-contaminated group (DON; n = 15), 3) DON-contaminated and M. hyopneumoniae-inoculated group (DONMHYO; n = 15), 4) M. hyopneumoniae-inoculated group (MHYO; n = 15). The piglets were fed the experimental diets ad libitum for five weeks and were monitored during this period and euthanized at day 35 [27 days post infection (DPI)] or 36 (28 DPI). The main parameters under investigation were macroscopic lung lesions (MLL) at euthanasia, respiratory disease score (RDS) from day 8 until day 35, histopathologic lesions and log copies of M. hyopneumoniae DNA detected by qPCR, determined at the day of euthanasia. Results: No significant difference was obtained for MLL at euthanasia, RDS (8-35), histopathologic lung lesions and log copies of M. hyopneumoniae DNA in the DONMHYO and MHYO group and consequently, no enhancement of the severity of the M. hyopneumoniae infection could be detected in the DONMHYO compared to the MHYO group. Conclusions: Under present conditions, the findings imply that feed contaminated with DON (1514 mu g/kg) provided to weaned pigs for five weeks did not increase the severity of an experimental M. hyopneumoniae infection. Further research is needed to investigate the impact of DON on M. hyopneumoniae infections in a multi-mycotoxin and multi-pathogen environment.
外文关键词：Mycoplasma hyopneumoniae； Experimental； Challenge； Clinical； Impact； Deoxynivalenol； Feed； Weaned piglets
作者：Michiels, A； Arsenakis, I； Matthijs, A； Boyen, F； Haesaert, G； Audenaert, K； Eeckhout, M； Croubels, S； Haesebrouck, F； Maes, D
作者单位：比利时根特大学
期刊名称：BMC VETERINARY RESEARCH
期刊影响因子：1.75
出版年份：2018
出版刊次：6
点击下载：脱氧雪腐镰刀菌烯醇（DON）3-乙酰基脱氧雪腐镰刀菌烯醇（3-A-DON）和15-乙酰脱氧雪腐镰刀菌烯醇（15-A-DON）对肺炎支原体感染猪只的影响研究