中文摘要：本研究组已有的研究表明，基于D肽Nap-G(D)F(D)F(D)Y的纳米纤维是一种比较好的疫苗佐剂。本研究表明，基于正向修饰的D-peptide Nap-G(D)F(D)F(D)Y(D)K形成的超分子水凝胶作为佐剂能够诱导更强烈的免疫反应。研究设计和合成了两种D-肽衍生物，一种包含正向修饰的(Nap-G(D)F(D)F(D)Y(D)K)，另一种包含负向修饰的(Nap-G(D)F(D)F(D)Y(D)E)。这两种衍生物都能通过纳米纤维形成水凝胶。结果表明，通过Nap-GDFDFDYDK形成的纳米纤维能够诱导产生比Nap-G(D)F(D)F(D)Y、Nap-G(D)F(D)F(D)Y(D)E更强烈的免疫反应。细胞试验表明，Nap-GDFDFDYDK形成的纳米纤维可以增强初级抗原呈递细胞对OVA的摄取；更重要的是Nap-GDFDFDYDK形成的纳米纤维在单剂量蛋白疫苗注射的情况下，能够保证机体维持持续的长达28周的高抗体滴度。该研究结果表明，基于正向修饰的D-peptide Nap-G(D)F(D)F(D)Y(D)K形成的超分子水凝胶是一种前景看好的疫苗佐剂，且能够应用于抗体生产和疫苗研发。
 
外文摘要：Self-assembling materials based on peptides have shown great potential as vaccine adjuvants. In our previous work, we have demonstrated that nanofibers based on D peptide Nap-G(D)F(D)F(D)Y are good candidates for vaccine " adjuvants. Here we further found that supramolecular hydrogels based on positively charged D-peptide Nap-G(D)F(D)F(D)Y(D)K as vaccine adjuvants could induce stronger immune response. We designed and synthesized two D -peptide derivatives, one with a positive charge (Nap-G(D)F(D)F(D)Y(D)K) and the other with a negative charge (Nap-G(D)F(D)F(D)Y(D)E). Both of them could form the hydrogels constructed by nanofibers. The nanofibers formed by Nap_Gr) DDnic FFypromoted the more powerful immune response in mice against the antigen chicken egg albumin (OVA) than peptides Nap-G(D)F(D)F(D)Y and Nap-G(D)F(D)F(D)Y(D)E. Through cell experiments, we demonstrated that the main reason was that nanofibers formed by Nap-GDFDFDYDK could enhance the uptake of OVA by primary antigen presenting cells. Most importantly, it was intriguing that the nonofibres based on Nap-G(D)F(D)F(D)Y(D)K could evoke long-lasting antibody titers for 28 weeks at a single dose of protein vaccine. Our study demonstrated that supramolecular hydrogels based on positively charged D-peptide were promising vaccine adjuvants and might be very useful for antibody production and vaccine development.
外文关键词：self-assembly； vaccine adjuvant； peptide； nanofiber
作者：Yang, CB； Shi, F； Li, C； Wang, YZ； Wang, L； Yang, ZM
作者单位：南开大学
期刊名称：ACS BIOMATERIALS SCIENCE & ENGINEERING
期刊影响因子：3.234
出版年份：2018
出版刊次：6
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