中文摘要：研究以比目鱼为对象，分析了IL-1 β、 IL-8、TNF-α和 G-CSF 4种重组细胞因子对迟钝爱德华菌亚单位疫苗rOmpV的佐剂效应。与对照组相比，rIL-1 β、 rIL-8和 rG-CSF能够显著增强血清特异性抗体水平和IgM + B淋巴细胞浓度，而rIL-1 β、 rIL-8诱导产生的抗体水平和淋巴细胞浓度显著高于rG-CSF。4种细胞因子均能诱导与体液免疫或细胞免疫相关的基因的表达，rIL-1 β、 rIL-8诱导产生的基因表达水平更高。同时，与对照组疫苗相对保护率40%相比，rOmpV+ rG-CSF、rOmpV+rIL-1 β、rOmpV+ rIL-8 组的相对保护率更高，分别为54%、75%和68%。该研究结果表明，rIL-1 β和rIL-8可作为抗迟钝爱德华菌亚单位疫苗的佐剂进行开发利用。
 
外文摘要：Cytokines have the potential as adjuvants for the application of vaccines in mammals. However, the adjuvant potential of teleost cytokines was limited. In the present work, the adjuvant effects of four recombinant cytokines including IL-1 beta, IL-8, TNF-alpha and G-CSF on E. tarda subunit vaccine rOmpV were comparatively investigated in flounder (Paralichthys olivaceus). Compared with control, the levels of specific serum antibodies and IgM + B lymphocytes were significantly enhanced by rIL-1 beta, rIL-8 and rG-CSF, whereas rIL-1 beta and rIL-8 induced significantly higher levels than rG-CSF. All four cytokines enhanced the expression of genes involved in humoral and/or cellular immunities, whereas rIL-1 beta and rIL-8 induced highest levels of genes involved in humoral immunities and cellular immunities, respectively. Compared to the relative percent survivals (RPS) of control group (40%) and rOmpV plus rG-CSF group (54%), rOmpV plus rIL-1 beta or rIL-8 produced higher RPS of 75% and 68%, respectively. Our results indicated that rIL-1 beta (3 and rIL-8 are promising adjuvants for subunit vaccines against E. tarda.
外文关键词：Cytokines； Adjuvant； Outer membrane protein V； Edwardsiella tarda； Paralichthys olivaceus； Immune response
作者：Guo, M； Tang, XQ； Sheng, XZ； Xing, J； Zhan, WB
作者单位：中国海洋大学
期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
期刊影响因子：2.913
出版年份：2018
出版刊次：9
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