中文摘要：本研究分析了肠道红嘴病疫苗与可溶性佐剂Montanide (TM) IMS 1312 VG PR、β-葡聚糖浸入式免疫鳟鱼对免疫反应的影响。其中，Montanide (TM) IMS 1312 VG PR单独使用时能够适当提高免疫保护作用（但作用不显著），而β-葡聚糖单独使用时不能增强免疫保护作用。佐剂免疫组与对照组在感染鲁氏耶尔森菌后血清溶菌酶活性升高。佐剂与疫苗联合使用时，特异性抗体浓度不受影响。腮腺中免疫相关基因的表达量高出表皮中表达量的许多倍，表皮中只有编码SAA 和 IL-17C1的基因表达量偏高。腮腺与表皮基因表达的动力学差异还表现编码细胞因子（TNF-alpha、 IL-1 beta、 IL-6、 IL-10、 IL-12、 IL-17A/F2、 IL-17C1、 IL-17C2、 IL-22）、免疫球蛋白类（IgM、IgD、IgT）、细胞标记（CD8 alpha、TCR-beta）和急性时相反应物（SAA、lysozyme）的基因表达上。与不使用佐剂相比，佐剂联合应用腮腺免疫组免疫24小时后，上述基因的表达量上调。该研究结果表明，佐剂加浸入式免疫能够增强疫苗的免疫反应，提高疫苗免疫保护作用及其持续期，但还需进一步的深入研究。
外文摘要：Immersion vaccination of rainbow trout against Yersinia ruckeri infection is an established method to prevent enteric red mouth disease (ERM) but the effect is inferior to injection vaccination and the duration of protection is limited to less than six months. Adjuvants in vaccines may in general elevate the immune response and the present work elucidates how ERM immersion vaccination of trout in combination with exposure to soluble adjuvants, Montanide (TM) IMS 1312 VG PR and beta-glucan, affects immune reactions. The former adjuvant, when used alone, induced a slightly increased protection ( not statistically significant) whereas beta-glucan did not increase protection. Adjuvant-treated and non-exposed groups showed elevated plasma lysozyme activity after challenge with Y. ruckeri. Specific antibody production was not positively affected by combining adjuvant and vaccine. Overall expression of immune genes tested was generally manifold higher in gills compared to skin. Only genes encoding SAA and IL-17C1 were expressed at a higher level in skin. Dynamic differences between the gill and skin compartments were also recorded for genes encoding cytokines (TNF-alpha, IL-1 beta, IL-6, IL-10, IL-12, IL-17A/F2, IL-17C1, IL-17C2, IL-22), immunoglobulins (IgM, IgD, IgT), cell markers (CD8 alpha, TCR-beta) and acute phase reactants (SAA, lysozyme). These genes were upregulated 24 h post-vaccination in fish gills exposed to both vaccine-adjuvant combinations when compared to fish exposed to vaccine alone. After a few weeks no vaccine induced reaction was seen and after challenge with bacteria mainly unvaccinated fish responded. Adjuvants used in combination with immersion vaccine clearly influences immune reactions and may improve duration and protection but further potency tests should be performed.
外文关键词：Yersinia ruckeri； Immersion vaccination； Adjuvant； Montanide (TM)； beta-glucan
作者：Skov, J； Chettri, JK； Jaafar, RM； Kania, PW； Dalsgaard, I； Buchmann, K
作者单位：丹麦哥本哈根大学
期刊名称：AQUACULTURE
期刊影响因子：2.57
出版年份：2018
出版刊次：7
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