中文摘要：本研究组先前的研究中构建了肺炎支原体的代谢模型，分别为猪鼻支原体代谢模型和絮状支原体代谢模型。前者病原毒性较小，但可引发疾病；后者为一种共生细菌。研究分析了这两种支原体的代谢差异，这些差异在一定程度上揭示了他们的不同毒性。其中一个重要性状就是，甘油代谢产生过氧化氢的现象只存在于能够引发疾病的支原体中；另一个重要特征是肺炎支原体肌醇的代谢路径。本研究对这2个特征进行了分析以阐明他们与支原体毒力差异的关系，比较分析包括可引起疾病的肺炎支原体和弱毒肺炎支原体。在肌醇代谢这一性状的分析中，研究表明当肌醇作为初始能量源时，只有肺炎支原体能够吸收碳水化合物且维持存活状态。在复合培养基中，只有两种致病肺炎支原体能够产生过氧化氢。研究同时表明，过氧化氢的产量取决于培养基中甘油的量。该研究揭示了与肺炎支原体毒力直接相关的2个重要代谢性状。
外文摘要：Mycoplasma hyopneumoniae is the causative agent of enzootic pneumonia. In our previous work, we reconstructed the metabolic models of this species along with two other mycoplasmas from the respiratory tract of swine: Mycoplasma hyorhinis, considered less pathogenic but which nonetheless causes disease and Mycoplasma flocculare, a commensal bacterium. We identified metabolic differences that partially explained their different levels of pathogenicity. One important trait was the production of hydrogen peroxide from the glycerol metabolism only in the pathogenic species. Another important feature was a pathway for the metabolism of myo-inositol in M. hyopneumoniae. Here, we tested these traits to understand their relation to the different levels of pathogenicity, comparing not only the species but also pathogenic and attenuated strains of M. hyopneumoniae. Regarding the myo-inositol metabolism, we show that only M. hyopneumoniae assimilated this carbohydrate and remained viable when myo-inositol was the primary energy source. Strikingly, only the two pathogenic strains of M. hyopneumoniae produced hydrogen peroxide in complex medium. We also show that this production was dependent on the presence of glycerol. Although further functional tests are needed, we present in this work two interesting metabolic traits of M. hyopneumoniae that might be directly related to its enhanced virulence.
 
外文关键词：MYCOIDES SMALL COLONY； HIDDEN MARKOV MODEL； FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE； GLYCEROL METABOLISM； ENZOOTIC PNEUMONIA； BINDS FIBRONECTIN； SWINE MYCOPLASMAS； SEQUENCE-ANALYSIS； COMPLETE GENOMES； RIBOSOMAL-RNA
作者：Ferrarini, MG； Mucha, SG； Parrot, D； Meiffrein, G； Bachega, JFR； Comte, G； Zaha, A； Sagot, MF
作者单位：法国 Inst Natl Rech Informat & Automat
期刊名称：MOLECULAR MICROBIOLOGY
期刊影响因子：3.898
出版年份：2018
出版刊次：6
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