中文摘要：研究以低分子量多聚胞苷酸即uPIC(40:400)为重点构建了一个弱毒RNA佐剂。试验将uPIC(40:400)与不同性状的金纳米粒子通过静电耦合。当与金纳米棒（非球形金纳米粒子）耦合时，能够显著增强uPIC(40:400)的佐剂特性，从而抑制小鼠的病毒感染。然而，uPIC(40:400)与金纳米棒耦合不能增加树突状细胞中炎性细胞因子的浓度。这些结果表明，金纳米棒能够提高uPIC(40:400)的佐剂活性，并维持较低的炎性细胞因子浓度。因此，这种金纳米棒耦合uPIC(40:400)佐剂可应用于鼻内灭活流感疫苗。
 
外文摘要：Intranasal inactivated influenza vaccines can elicit mucosal immune responses that protect against virus infection. For the development of intranasal inactivated influenza vaccines, effective adjuvants inducing minimal adverse reactions are required. Generally, however, lower toxicity adjuvants have lower adjuvanticity. In this research, we fabricated nanoparticle-based adjuvants to enhance its adjuvanticity. Herein, we focused on low-molecular-weight polyinosinic-polycytidylic acid, referred to as uPIC(40:400), as a weak and less toxic RNA adjuvant. We conjugated uPIC(40:400) with different shaped gold nanoparticles (AuNPs) electrostatically. Conjugation with gold nanorods, but not spherical AuNPs, markedly enhanced the adjuvanticity of uPIC(40:400), leading to the suppression of viral infection in mice. Notably, conjugation with gold nanorods did not increase the inflammatory cytokine production in dendritic cells. These data indicated that gold nanorods can provide a good platform for enhancing the weak adjuvanticity of uPIC(40:400) while maintaining low inflammatory cytokine production toward the development of intranasal inactivated influenza vaccines.
外文关键词：HIGH-YIELD SYNTHESIS； DOUBLE-STRANDED-RNA； GOLD NANOPARTICLES； CELLULAR UPTAKE； AQUEOUS-SOLUTION； DENDRITIC CELLS； VIRUS INFECTION； IN-VIVO； SIZE； NANORODS
作者：Tazaki, T； Tabata, K； Ainai, A； et al.
作者单位：日本传染病研究所
期刊名称：RSC ADVANCES
期刊影响因子：3.06
出版年份：2018
出版刊次：8
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