中文摘要：研究构建了一个基于表面增强拉曼散射的侧向流免疫分析方法用于检测人类血清中肺炎支原体。基于表面增强拉曼散射的侧向流免疫分析试纸采用Au@Ag纳米粒子（Au@Ag NPs）装载2层拉曼染料5,5'-二硫双（2-硝基苯甲酸）（DTNB）作为表面增强拉曼散射探针(SERStags)。Au/DTNB@Ag/DTNB的优点是在检测抗体方面据哟高度敏感性和生物共轭柔性。SERS-LFIA试纸通过监测SERS信号对人类IgM的浓度进行定量。该方法对IgM的检测限为0.1 ng/ mL，其灵敏度是比色法的100倍以上。试验对20份 样本进行了检测，对阳性样本检测的精准度达到了100%，而平行酶联免疫吸附试验的检测率只有85%。SERS-LFIA试纸的特异性较好，能够进行临床应用。
 
外文摘要：We present an attractive model of surface-enhanced Raman scattering-based lateral flow immunoassay (SERS-LFIA) for the sensitive and accurate detection of Mycoplasma pneumoniae (MP) infection in human serum. The SERS-LFIA strip uses Au@Ag nanoparticles (Au@Ag NPs) loaded with two layers of Raman dye 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) as SERS tags. The advantages of the dual dye-loaded SERS tags (Au/DTNB@Ag/DTNB) are the high sensitivity and the bioconjugation flexibility of the detection antibody. As determined from our SERS-LFIA strip, human IgM was quantified by monitoring the SERS signal on the test line. The limit of detection for human IgM was 0.1 ng mL(-1), which was 100 times more sensitive than that by using the colorimetric method. Our assay results for 20 MP-specific IgM positive serum specimens showed 100% accuracy and detection rate, whereas the parallel enzyme-linked immunosorbent assay only showed 85% detection rate. The SERS-LFIA strip also exhibited high specificity and potential clinical applications. Therefore, our SERS-based LFIA strip has strong potential for practical applications in the sensitive and rapid detection of MP.
外文关键词：ENHANCED RAMAN-SCATTERING； GOLD NANOPARTICLES； RAPID DETECTION； DIAGNOSIS； STRIP； SPECTROSCOPY； ASSAY； IGM； IMMUNOCHROMATOGRAPHY； AMPLIFICATION
作者：Jia, XF； Wang, CW； Rong, Z； Li, J； Wang, KL； Qie, ZW； Xiao, R； Wang, SQ
作者单位：北京工业大学
期刊名称：RSC ADVANCES
期刊影响因子：3.06
出版年份：2018
出版刊次：8
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