中文摘要：研究评估了霍乱弧菌神经氨酸（NA）作为PLGA微粒（MPs）的一种新型上皮靶向分子在提高粘膜变态反应免疫疗法方面的作用，并与已有的上皮细胞靶向凝集素小麦胚芽凝集素（WGA）和橙黄网胞盘菌凝集素（AAL）进行比较。结果所有的靶向靶向因子均能与Caco-2细胞结合，但只有NA与α-L海藻糖和单唾液酸神经节苷酯-1具有较高的结合特异性。在M-细胞共培养模型中，NA-MPs经上皮摄取的比例增加。NA和NA-MPs能够诱导小鼠脾细胞产生高浓度的IFN-g 和IL10，诱导Caco-2中CCL20的表达。NA-MPs重复口服会形成一种新的经过调节的变应原特异性免疫反应。总而言之，NA与其它靶向因子相比能够增强M细胞特异性。NA功能性MPs诱导产生一种Th1和T-调控性驱动免疫反应，且避免了变态反应细胞的活化，作为一种新型的口服变态反应疗法具有一定的应用前景。
 
外文摘要：To improve current mucosal allergen immunotherapy Vibrio cholerae neuraminidase (NA) was evaluated as a novel epithelial targeting molecule for functionalization of allergen-loaded, poly(D,L-lactide-co-glycolide) (PLGA) microparticles (MPs) and compared to the previously described epithelial targeting lectins wheat germ agglutinin (WGA) and Aleuria aurantia lectin (AAL). All targeters revealed binding to Caco-2 cells, but only NA had high binding specificity to alpha-L fucose and monosialoganglioside-1. An increased transepithelial uptake was found for NA-MPs in a M-cell co-culture model. NA and NA-MPs induced high levels of IFN-gamma and IL10 in naive mouse splenocytes and CCL20 expression in Caco-2. Repeated oral gavage of NA-MPs resulted in a modulated, allergen-specific immune response. In conclusion, NA has enhanced M-cell specificity compared to the other targeters. NA functionalized MPs induce a Th1 and T-regulatory driven immune response and avoid allergy effector cell activation. Therefore, it is a promising novel, orally applied formula for allergy therapy.
外文关键词：Neuraminidase； M-cell； Oral application； PLGA-microparticles； AAL； WGA
作者：Diesner, SC；Bergmayr, C；Wang, XY；Heiden, D；Exenberger, S；Roth-Walter, F；Starkl, P；Ret, D；Pali-Scholl, I；Gabor, F；Untersmayr, E
作者单位：澳大利亚维也纳医科大学
期刊名称：CLINICAL IMMUNOLOGY
期刊影响因子：3.557
出版年份：2018
出版刊次：8
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