中文摘要：本研究组已有的研究表明，大多数肺炎支原体肺炎患者血浆总免疫球蛋白E（IgE）的水平升高。本研究目的在于分析肺炎支原体中导致儿童IgE浓度增高的成分，并对其特殊机制进行分析。结果表明：肺炎支原体肺炎儿童血浆中能够检测到P1特异性IgE，这种P1蛋白的分子量约为24-kDa。约9.2%的肺炎支原体肺炎患儿能够产生抗P1蛋白多肽的IgE，这部分患儿大都无过敏史或家族性过敏性疾病史。P1特异性IgE阳性的肺炎支原体肺炎患者表现出更为严重的临床症状，其IL-4和IL-5 两种细胞因子过度分泌、Th0细胞向Th2细胞的分化过度。同时，P1蛋白刺激了健康献血者外周血外周血单核（细胞）的过度分泌。因此，肺炎支原体不仅是一种传染源，也是部分人群的一种过敏原。肺炎支原体的P1蛋白能够诱导产生P1特异性IgE。
 
外文摘要：Background: Our previous study found that most Mycoplasina pneurnoniae (MP) pneumonia (MPP)patients had elevated serum total immunoglobulin E (IgE) levels. Objective: To determine components of MP that can cause an IgE increase in children, and to clarify its specific mechanism. Methods: The components of MP cells were isolated by serum IgE from patients with MP pneumonia. These components obtained through the prokaryotic expression were used as allergens to detect the proportion of allergen-specific IgE produced in MPP patients, and the clinical characteristics and related immune parameters of these patients who produced this allergen-specific IgE were also analyzed. In addition, a cell experiment was used to verify the biological effect of these components in vitro. Results: Pl-specific IgE was detected in serum of MPP children. An approximately 24-kDa polypeptide of P1 protein was obtained through prokaryotic expression purified by nickel agarose affinity chromatography. Approximately 9.2% of MPP patients produced IgE against this polypeptide of P1 protein, which was more likely to be produced in MPP patients with no history of allergies or family history of allergy-related diseases. P1-specific IgE-positive MPP patients had more severe clinical symptoms, with excessive secretion of interleukin (IL)-4 and IL-5 and overdifferentiation of Th0 cells into Th2 cells. Tests also demonstrated that the P1 protein stimulated excessive secretion of IL-4 and IL-5 in peripheral blood mononuclear cells from the peripheral blood of healthy donors. Conclusion: Mycoplasnia pneuntonine is not only an infectious agent but also an allergen for certainindividuals. The P1 protein of MP can induce the production of Pl-specific IgE.
外文关键词： ASTHMA；CHILDREN；INFECTION；ILLNESS；WHEEZE；ONSET；CELLS
作者：Ye； Qing; Mao； Jian-hua; Shu； Qiang; Shang； Shi-qiang
作者单位：浙江大学
期刊名称：ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY
期刊影响因子：3.263
出版年份：2018
出版刊次：7
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