中文摘要：研究建立了一种新型的实时定量检测肺炎支原体、猪鼻支原体和絮状支原体的方法，分析了支原体类型与肺炎样皮损程度的关系。试验采集和分析了671个样本，肺炎支原体、猪鼻支原体和乳状支原体的检测率分别为59.5%、34%和34.7%，支原体基因组当量分别为31x107、 97x106和57x106；肺部无病变或病变部位较小的样本多重PCR检测结果为阴性或者絮状支原体阳性。肺部广泛性病变的样本PCR检测结果为肺炎支原体或猪鼻支原体阳性。该结果表明，肺炎支原体和猪鼻支原体显著提高了肺炎的感染程度。肺部病变与支原体基因组当量不相关。研究建立的一种新型多重qPCR法特异性、敏感性好，可重复性强。
 
外文摘要：Aims A new multiplex qPCR, targeting Mycoplasma (M.) hyopneumoniae, M. hyorhinis and M. flocculare, was developed and the relationship between detection of those mycoplasma species and the extent of gross pneumonia-like lesions in slaughtered pigs lungs were investigated. Methods and ResultsThe multiplex qPCR method targets the p102, p37 and fruA genes and has detection limits of 14, 146 and 16 genome equivalents l(-1) for M. hyopneumoniae, M. hyorhinis and M. flocculare, respectively. In all, 671 lungs were collected and analysed, among them 666 were scored for macroscopic pneumonia and categorized according to the extent of the lesions (no or minor lesions, moderate lesions and extensive lesions). According to results of multiplex qPCR, 595% were positive for M. hyopneumoniae, 34% for M. hyorhinis and 347% for M. flocculare, with on average, 31x10(7), 97x10(6) and 57x10(6) genome equivalents of mycoplasma ml(-1), respectively. More results showed that no or minor lesions were associated with multiplex qPCR-negative results or qPCR-positive results for M. flocculare. Moderate to extensive lesions were positively correlated with qPCR-positive results for M. hyopneumoniae. Extensive lesions were associated with qPCR-positive results for at least two mycoplasma species (M. hyopneumoniae and M. hyorhinis). ConclusionThe findings also indicated that M. hyopneumoniae and M. hyorhinis significantly increased the odds for a lung to have macroscopic pneumonia. No relationship was found between the extent of lesions and the mycoplasma genome load. Significance and Impact of the studyThis new multiplex qPCR appears to be specific, sufficiently sensitive and repeatable. The validation of this method with field samples guarantees its use for field epidemiological investigations, particularly to gain more insight into the aetiology of the porcine respiratory disease complex.
外文关键词：detection； diseases； epidemiology； infection； PCR (polymerase chain reaction)
作者：Fourour, S； Fablet, C； Tocqueville, V； Dorenlor, V； Eono, F； Eveno, E； Kempf, I； Marois-Crehan, C
作者单位：法国食品环境与职业安全机构
期刊名称：JOURNAL OF APPLIED MICROBIOLOGY
期刊影响因子：2.156
出版年份：2018
出版刊次：8
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