中文摘要：研究分析了Pam3CSK4和成纤维细胞刺激脂肽-1（FSL-1）对肠病毒71粘膜疫苗的佐剂作用。试验以BALB/c裸鼠为对象进行鼻内免疫3次，每次间隔3周，结果表明：EV71 + FSL-1免疫组血清、鼻洗液、支气管肺泡灌洗液和粪便中EV71特异性IgG和IgA浓度显著高于EV71 + Pam3CSK4、EV71免疫组和磷酸盐缓冲液对照组。同时，EV71 + FSL-1免疫组分泌EV71-特异性IgG和IgA抗体的细胞增多，T细胞增殖反应、IFN-g和IL-17的分泌量显著升高。此外，免疫小鼠血清能够中和EV71（C2基因型），能够交叉中和B4和B5基因型EV71。该研究结果表明，FSL-1是鼻内免疫EV71疫苗的有效佐剂。
 
外文摘要：To prevent viral infection at the site of entry, mucosal vaccines are potent tools for inducing IgA secretion for defense. Because Toll-like receptor (TLR) ligands serve as strong adjuvants, two ligands that mimic the structure of mycoplasmal and bacterial lipopeptides represent interesting vaccine candidates. Pam3CSK4, a synthetic triacylated lipopeptide, interacts with TLR2/1. Because fibroblast-stimulating lipopeptide-1 (FSL-1), a synthetic diacylated lipopeptide, is recognized by TLR2/6, we targeted the potential immuno-inducibility of Pam3CSK4 and FSL-1 as adjuvants of an enterovirus 71 (EV71) mucosa] vaccine. Naive BALB/c mice were used for intranasal immunization three times over a 3-week interval, with results showing that EV71-specific IgG and IgA in serum, nasal washes, bronchoalveolar lavage fluid, and feces from the EV71 + FSL-1 group were significantly higher than levels observed in mice treated with EV71 + Pam3CSK4, EV71 alone, or the control group treated with phosphate-buffered saline. Furthermore, we observed more EV71-specific IgG and IgA-producing cells in treatments using EV71 formulated with FSL-1. Additionally, T cell-proliferative responses and interferon-gamma and interleukin-17 secretion were significantly increased when inactivated EV71 was formulated using FSL-1. Moreover, serum from immunized mice was capable of neutralizing the infectivity of EV71 (C2 genotype) and was able to cross-neutralize the B4 and B5 genotypes of EV71. Our data suggested that FSL-1 could be used as an efficient adjuvant for intranasal EV71-vaccine immunization.
外文关键词：Adjuvant； Enterovirus 71； FSL-1； IgA； Mucosal vaccine； Pam3CSK4
作者：Lin, YL； Cheng, PY； Chin, CL； Huang, LM； Lin, SY； Chiang, BL
作者单位：中国台湾国立台湾大学医学院
期刊名称：VACCINE
期刊影响因子：2.229
出版年份：2018
出版刊次：7
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