中文摘要：研究采用鞭毛为佐剂的PED疫苗rSF-COE-3D注射生长猪的后海穴，显著增强了血清IgG/IgA抗体反应、提高了粘膜IgA和血清中和抗体浓度，提高了IFN-g和IL-4的浓度。rSF-COE-3D可以提高猪群抗高致病性PEDV变异体的能力；免疫后腹泻猪只、检测到PEDV阴影的猪只数量减少，PEDV感染猪只粪便病毒指数、十二指肠表观病变和炎症反应降低。rSF-COE-3D疫苗提高了猪群的保护效率，主要体现在血清IgA抗体浓度、粪便粘膜IgA抗体浓度和血清中和抗体滴度的提高。总而言之，研究表明rSF-COE-3D疫苗是一种新型、高效的PED疫苗。
 
外文摘要：PEDV variants, causing severe diarrhea in neonatal suckling piglets with a mortality rate up to 100%, have being epidemic since late 2010 in china. To meet the pressing need of safe and cost-efficient PED maternal vaccines against PEDV variant, we vaccinated growing piglets with a flagellin-adjuvanted PED vaccine rSF-COE-3D by injection at Houhai acupoint. The vaccination not only enhanced the antibody responses of serum IgG/IgA, mucosal IgA and serum neutralizing antibody, but also improved the production of IFN-gamma and IL-4. Moreover, rSF-COE-3D could provide a better protection against the challenge of a high pathogenic PEDV variant, with less diarrhea pigs, less pigs with detectable PEDV shed, lower rank values of feces and less apparent lesions and inflammation in duodenum of the PEDV infected pigs. The improved protective efficiency of rSF-COE-3D compared with COE was mostly benefited from the enhanced production of serum IgA, mucosal IgA in feces and serum neutralizing antibody titers. Taken together, our data suggest that rSF-COE-3D would be a novel efficient PED vaccine.
外文关键词：Porcine epidemic diarrhea； Flagellin； Subunit vaccine； Protective efficiency
作者：Li, QN； Xu, ZC； Wu, TT； Peng, OY； Huang, LC； Zhang, Y； Xue, CY； Wen, ZF； Zhou, QF； Cao, YC
作者单位：中山大学
期刊名称：VACCINE
期刊影响因子：2.229
出版年份：2018
出版刊次：7
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