研究利用兔免血清特异性识别鉴定猪肺炎支原体蛋白，结果表明：絮状支原体和肺炎支原体之间存在交叉反应，这与前人通过ELISA试验获得的结果一致。39个肺炎支原体蛋白中，只有mhp182（42-kDa C端片段）、mhp638 和 mhp684（C端片段）等3个蛋白没有表现出与猪其它支原体蛋白的反应。蛋白mhp384和mhp511与肺炎血清反应对3种支原体产生抗性；抗猪鼻支原体、肺炎支原体血清不与肺炎支原体蛋白反应。这些结果表明，由于不同支原体蛋白存在交叉反应现象，不显著的絮状支原体感染也会导致肺炎支原体血清检验呈阳性反应，而滑液支原体感染导致血清检验阳性反应的可能性较低，猪鼻支原体感染不会影响血清检验结果。
外文摘要：Mycoplasmas are cell wall-less bacteria that infect a variety of animals in a species-specific manner. In swine, Mycoplasma hyopneumoniae is the most virulent and presents the most disease and economic problems to the swine industry. Serological assays are commonly used to assess colonization and disease, but antigenic cross-reactivity between M. hyopneumoniae and other mycoplasma species, most notably Mycoplasma hyorhinis, Mycoplasma hyosynoviae and Mycoplasma flocculare, is a concern. The extent of cross-reactivity has not been thoroughly investigated. These studies were designed to identify M. hyopneumoniae proteins that are recognized by rabbit hyperimmune sera raised against the other swine mycoplasmas. Our results indicate extensive cross-reactivity between M. flocculare and M. hyopneumoniae, which explains previous reports seen with ELISA assays. Only three of the thirty-nine M. hyopneumoniae proteins tested showed no cross reactivity with the other three swine mycoplasmas, mhp182 (42kDa C-terminal fragment), mhp638 and mhp684 (C-terminal fragment). Two proteins, mhp384 and mhp511, were cross-reactive with hyperimmune sera generated against three of the four species. None of the anti-M. hyorhinis hyperimmune sera reacted to any of the M. hyopneumoniae proteins. These results suggest that inapparent M. flocculare infections could produce positive responses in M. hyopneumoniae serological assays due to cross-reactivity, and that M. hyosynoviae infections are less likely to do so and M. hyorhinis infections unlikely to affect assay results.
外文关键词：Cross-reactive antigens；Protein microarrays；Serology；Swine mycoplasmas
作者：Petersen AC, Oneal DC, Seibel JR等，
作者单位：美国爱荷华州立大学。
期刊名称：VETERINARY MICROBIOLOGY
期刊影响因子：2.564
出版年份：2016
出版刊次：7
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