中文摘要：本研究的目的在于探索感染支原体肺炎的儿童急性气喘的影响因素。试验以感染了支原体肺炎的84个儿童为对象，分别将其分为气喘组（40人）和非气喘组（40人），利用RT-PCR技术检测了试验对象的T细胞免疫球蛋白域粘蛋白基因（Tim）1,3和Toll样受体2,4的表达情况，应用酶联免疫法测定了血浆中IL-10、TNF-α、IFN-γ和Ig的浓度，利用自动血液仪进行外周血嗜酸性粒细胞计数。结果表明：感染了支原体肺炎的儿童体内TLR2、TLR4、Tim1、IL-10、TNF-α、IgE和EOS水平显著高于健康对照组，IFN-γ水平低于对照组；其中感染组的气喘儿童体内TLR2、Tim1、Tim3、TNF-α、IgE和EOS水平显著高于非气喘儿童；气喘儿童支原体特异性抗体滴度与TLR2、TIM1水平呈正相关，而与IFN-γ呈负相关。IgE水平与TLR2、TLR4 和 Tim1水平呈正相关，EOS与Tim1、Tim3呈负相关。该结果表明，TLR2、Tim1、Tim3、TNF-α、IgE和EOS对儿童支原体肺炎相关的气喘症状具有一定的影响；其中IgE的作用与TLR2、Tim1相关，EOS的作用与Tim1、Tim3相关。
外文摘要：Background The study aimed to explore possible factors influencing wheezing in children with Mycoplasma pneumoniae pneumonia (MPP). Methods The study included 84 children with MPP, who were divided into two groups: wheezy group (n = 40) and non-wheezy group (n = 44), along with 30 age-matched healthy controls. T-cell immunoglobulin and mucin domain gene (Tim) 1, 3 and Toll-like receptor (TLR) 2, 4 were evaluated using RT-PCR. Serum IL-10, TNF-alpha, IFN-gamma and IgE were assessed by enzyme-linked immunosorbent assay. Peripheral blood eosinophil (EOS) was measured by an automated haematology. Results Children with MPP had markedly increased TLR2, TLR4, Tim1, IL-10, TNF-alpha, IgE and EOS, and decreased IFN-gamma than the healthy controls. In the presence of MPP, wheezy children had significantly elevated TLR2, Tim1, Tim3, TNF-alpha, IgE and EOS than non-wheezy children. In wheezy children with MPP, MP-specific antibody titre was positively correlated with TLR2 and TIM1, and negatively correlated with IFN-gamma. IgE was positively correlated with TLR2, TLR4 and Tim1, while EOS was positively correlated with Tim1 and Tim3. Conclusion TLR2, Tim1, Tim3, TNF-alpha, IgE and EOS play a role in MPP-related wheezing in children. The role of IgE might be associated with TLR2 and Tim1, and the role of EOS might be associated with Tim1 and Tim3.
外文关键词：Inflammatory factors；Mycoplasma pneumoniae；T-cell immunoglobulin；Wheeze
作者：Fan, Q; Gu, TT; Li, PJ; Yan, P; Chen, DH； Han, BC
作者单位：济南市妇幼保健院
期刊名称：HEART LUNG AND CIRCULATION
期刊影响因子：1.575
出版年份：2016
出版刊次：12
点击下载：T细胞免疫球蛋白域粘蛋白基因和Toll样受体在支原体肺炎儿童急性气喘中的作用