中文摘要：本研究对接种含有AS03佐剂的流感疫苗、不含佐剂的疫苗以及季节性流感疫苗后血清细胞B细胞受体进行了高通量测序试验，以阐明佐剂对体液免疫的影响。结果表明，佐剂主要通过两种机制发挥作用。一是增强初始B细胞的活性，从而减少先前接种过的疫苗的免疫干扰；二是增强所激活细胞的适应性从而提高细胞对新注射疫苗中抗原的特异性。因此，AS03佐剂增强了pH1N1的免疫反应，降低了免疫干扰。
外文摘要：Influenza pandemics require rapid deployment of effective vaccines for control. Adjuvants such as AS03 improve vaccine immunogenicity, but this mechanism is poorly understood. We used high-throughput B cell receptor sequencing of plasma cells produced following AS03-adjuvanted and non-adjuvanted 2009 pandemic H1N1 vaccination, as well as pre-pandemic seasonal influenza vaccination to elucidate the effect of the adjuvant on the humoral immune response. By analyzing mutation levels, it was possible to distinguish sequences from cells that were recently activated from naive B cells from those that were activated by memory recall. We show that the adjuvant functions through two mechanisms. First, the adjuvant stimulates increased activation of naive B cells, thus reducing immune interference with previous vaccine responses. Second, the adjuvant is able to increase the adaptability of the recalled cells to give improved specificity to the new vaccine antigen. We thus show how AS03 enhances pH1N1 immune responses, and reduces immune interference.
外文关键词：PANDEMIC H1N1 INFLUENZA; MONOCLONAL-ANTIBODIES; RESPONSES; HUMANS; MEMORY; VIRUS; MECHANISM; SYSTEM
作者：Galson, JD; Truck, J; Kelly, DF; van der Most, R
作者单位：牛津大学
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：5.228
出版年份：2016
出版刊次：11
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