中文摘要：研究建立了一种衣阿华支原体的多位点分型技术，共检测到看家基因的26个位点，并对其进行了研究。筛选其中6个基因进一步进行多位点分型检测，序列分析结果将47个衣阿华支原体样本分成20个不同的类型。系统发生树和聚类分析结果与样本的地理分布、时空信息等吻合度较高。因此多位点分型技术（MLST）是一种重复性较高的分析流行病学诊断技术，可直接用于鉴定临床阳性样本。
 
外文摘要：Mycoplasma iowae (MI) infection is an economically and commercially important disease of turkeys. There are no sequence typing assays available for MI strain identification, the only available molecular tools for this purpose, are DNA fingerprinting assays. In addition to their low reproducibility, fingerprinting assays require isolation of the microorganism in pure culture, which is difficult for avian mycoplasma. Therefore, we propose a multilocus sequence typing (MLST) assay as the first genotyping assay for identification of MI. Based on the two available MI genomes on GenBank, 26 loci of housekeeping genes were identified and studied in a diverse sample set. Finally, six genes were selected for the newly developed MLST assay. The final sequence analysis of the six loci (total of 5019 bp) (dppC, ulaA, valS, rpoC, leuS, kdpA) allowed the differentiation Of 47 MI samples into 23 unique-sequence types. Moreover, when only 4 loci were used to type the same set of samples, they resulted in 20 unique sequence types. Analysis of phylogenetic trees and clonal groups generated by MLST displayed a high degree of agreement with geographical and temporal information of the tested samples. MLST is a highly reproducible molecular epidemiology assay that can be used to identify positive clinical cases directly from DNA samples. Therefore, it provides a useful tool allowing for better identification, control and eradication efforts.
外文关键词：MLST; Mycoplasma iowae; Turkeys; Molecular-typing
作者：Ghanem, M ; El-Gazzar, M
作者单位：俄亥俄州立大学
期刊名称：VETERINARY MICROBIOLOGY
期刊影响因子：2.564
出版年份：2016
出版刊次：11
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