中文摘要：研究分析了由HER2和IL-12组成的重组DNA疫苗对小鼠直肠癌的作用及其免疫机制。试验以表达HER2的肿瘤小鼠为模型。结果表明：小鼠接种HER2-IL-12质粒后对HER2-诱导的肿瘤的生长起到了显著的抑制作用，延长了小鼠的寿命。该结果表明，IL-12在DNA疫苗佐剂应用方面具有较大的潜力，基于HER2和IL-12的疫苗未来能够用于治疗直肠癌。
外文摘要：The present study aimed to determine the effect of recombinant DNA vaccine-based human epidermal growth factor receptor-2 (HER2) and Interleukin 12 (IL-12) on the development of colonic carcinoma in mice and the potential immune mechanisms involved. Recombinant plasmids pVAX1-HER2, pVAX1-IL-12 and pVAX1-HER2-IL-12 were constructed, and injected into female mice intramuscularly (i.m.) followed by an electric pulse. The humoral and cellular immune responses after immunization were examined by enzyme linked immunosorbent assay (ELISA) and enzyme-linked immunospot assay (ELISPOT), respectively. To evaluate the anti-tumor efficacy of the plasmids, a mouse model with a HER2-expressing tumor was designed. Mice vaccinated with the HER2-IL-12 plasmid generated the strongest inhibition efficacy on the growth of HER2-expressing tumors and prolonged mouse survival. These observations emphasized the potential of IL-12 as an adjuvant for DNA vaccines and of vaccines based on HER2 and IL-12 as a promising treatment for colonic carcinoma.
 
外文关键词：Colonic carcinoma；HER2；IL-12；anti-tumor； DNA vaccine
作者：Gao, P; Zhang, CH; Bian, XX; Guo, YJ; Wei, YG; Zhang, L; Liu, ZY; Wang, XY; Huang, SM
作者单位：大庆油田总医院
期刊名称：IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
期刊影响因子：1.617
出版年份：2016
出版刊次：12
点击下载：由HER2和佐剂IL-12组成的结肠癌DNA疫苗的抗肿瘤效果研究