中文摘要：研究分析了疫苗佐剂CIA06对接种H1N1流感疫苗Greenflu-S的小鼠的作用。试验构建了一个佐剂系统，该系统由toll样受体4促进剂de-O-酰化脂低聚糖（dLOS）和氢氧化铝组成。结果表明：CIA06显著提高了流感特异性血清Ig G、血凝抑制和病毒抑制抗体滴度，从而消除了疫苗对抗体反应的剂量效应。CIA06传递的Greenflu-S疫苗提高了小鼠Th1主导的细胞因子浓度，诱导了CD4(+) 和 CD8(+) T细胞反应。CIA06传递的Greenflu-S疫苗降低了小鼠的死亡率以及感染流感病毒后的死亡率，且病毒感染后未见小鼠出现肺部的过度炎症反应。这些结果表明，CIA06可作为流感疫苗的佐剂应用时可促进机体免疫反应，也可替代部分剂量的抗原而不会导致严重的炎症反应。
 
外文摘要：Vaccine adjuvants are agents that are used to promote immune responses to vaccine antigens and thereby to enhance the protective efficacy of the vaccines. In this study, we investigated the adjuvant activity of CIA06, an adjuvant system that is composed of a toll-like receptor 4 agonist de-O-acylated lipooligosaccharide (dLOS) and aluminum hydroxide, on the H1N1 pandemic influenza vaccine Greenflu-S (R) in mice. CIA06 significantly enhanced influenza-specific serum IgG, hemagglutination-inhibition, and virusneutralizing antibody titers, which eliminated vaccine dose-dependency in the antibody response. Mice immunized with the CIA06-adjuvanted Greenflu-S showed Th1-type-predominant cytokine profiles, and both CD4(+) and CD8(+) T cell responses were induced. Immunization of mice with the CIA06-adjuvanted vaccine reduced the mortality and morbidity of mice upon lethal challenges with influenza virus, and no excessive inflammatory responses were observed in the lung tissues of the immunized mice after viral infection. These data suggest that the dLOS-based adjuvant system CIA06 can be used to promote the immune responses to influenza vaccine or to spare antigen dose without causing harmful inflammatory responses.
 
外文关键词：RESPIRATORY SYNCYTIAL VIRUS; ANTHRACIS PROTECTIVE ANTIGEN; MONOPHOSPHORYL-LIPID-A; COLI DNA FRAGMENTS; ACUTE LUNG INJURY; MODIFIED LIPOPOLYSACCHARIDES; ELDERLY ADULTS; TLR4 AGONIST; HPV VACCINE; COMBINATION
作者：Ryu, Ji In; Park, Shin Ae; Wui, Seo Ri; Ko, Ara; Han, Ji Eun; Choi, Jung Ah; Song, Man Ki; Kim, Kwang Sung; Cho, Yang Je; Lee, Na Gyong
作者单位：韩国世宗大学
期刊名称：BIOMED RESEARCH INTERNATIONAL
期刊影响因子：2.134
出版年份：2016
出版刊次：12
点击下载：一种De-O酰化脂低聚糖佐剂系统及其对接种H1N1流感病毒疫苗小鼠抗体产生和Th1-型免疫反应激活的作用