中文摘要：本研究分析了20个肺炎支原体菌株的全基因序列，其中3个为已公开的菌株，3个分离自无明显症状的儿童，13个分离分离自临床出现明显呼吸道感染症状的病人，1个分离自呼吸道感染康复的病人。结果表明，分别分离自无明显症状和有临床症状的支原体菌株形成2个不同的类群，亚型1和亚型2。研究揭示了分离自有临床症状的菌株与已公开的菌株具有明显的遗传差异，这一结果表明就目前群体中流行的肺炎支原体而言，已公开的菌株不具代表性。
外文摘要：Mycoplasma pneumoniae is a common cause of respiratory tract infections (RTIs) in children. We recently demonstrated that this bacterium can be carried asymptomatically in the respiratory tract of children. To identify potential genetic differences between M. pneumoniae strains that are carried asymptomatically and those that cause symptomatic infections, we performed whole-genome sequence analysis of 20 M. pneumoniae strains. The analyzed strains included 3 reference strains, 3 strains isolated from asymptomatic children, 13 strains isolated from clinically well-defined patients suffering from an upper (n = 4) or lower (n = 9) RTI, and one strain isolated from a follow-up patient who recently recovered from an RTI. The obtained sequences were each compared to the sequences of the reference strains. To find differences between strains isolated from asymptomatic and symptomatic individuals, a variant comparison was performed between the different groups of strains. Irrespective of the group (asymptomatic vs. symptomatic) from which the strains originated, subtype 1 and subtype 2 strains formed separate clusters. We could not identify a specific genotype associated with M. pneumoniae virulence. However, we found marked genetic differences between clinical isolates and the reference strains, which indicated that the latter strains may not be regarded as appropriate representatives of circulating M. pneumoniae strains.
外文关键词：Mycoplasma pneumoniae;colonization;infection;respiratory tract infections;whole genome sequencing
作者：Spuesens, EBM; Brouwer, RWW; Mol, KHJM; Hoogenboezem, T; Kockx, CEM; Jansen, R; Van IJcken, WJ; Van Rossum, AMC; Vink, C
作者单位：新西兰鹿特丹Erasmus MC索菲亚儿童医院
期刊名称：FRONTIERS IN MICROBIOLOGY
期刊影响因子：5.218
出版年份：2016
出版刊次：10
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