中文摘要：本研究评价了3种佐剂（氢氧化铝、Essai微球和Phema纳米颗粒）增强鸡H6N2禽流感DNA疫苗免疫的作用。试验组和对照组（无免疫佐剂的pCAG-HAK疫苗）均未检测到血凝抑制抗体。结果表明：与空白对照组相比，无佐剂疫苗组和Essai佐剂组口咽和泄殖腔拭子病毒脱落的频次显著降低；而氢氧化铝组和Phema组口咽拭子的病毒脱落频次显著降低，该结果表明这些佐剂能够进一步增强病毒的效力。氢氧化铝由于价格低廉、安全，能够增强鸡DNA疫苗诱导的免疫反应，可以作为一种理想的佐剂进行推广。
 
外文摘要：This study assessed the ability of three adjuvants (aluminium hydroxide, Essai (microparticle) and Phema (nanoparticle)) to enhance the immune response of chickens to an H6N2 avian influenza DNA vaccine. No haemagglutination inhibition antibody was detected following two intramuscular immunizations with the adjuvanted and non-adjuvanted pCAG-HAk vaccine, which has previously been shown to induce moderate H6 haemagglutinin antibody response in SPF chickens. Following virus challenge, neither the vaccinated group without adjuvant nor the Essai-adjuvanted group showed a statistically significant reduction in virus shedding in oropharyngeal and cloacal swabs compared with the naive control group. However, the aluminium hydroxide and Phema-adjuvanted groups significantly reduced the frequency of virus shedding in oropharyngeal swabs, indicating that these adjuvants appeared to further enhance the vaccine potency. Aluminium hydroxide holds promise as an adjuvant for enhancing DNA-induced immune response in chickens owing to its low price and safety record.
 
外文关键词：Adjuvant;DNA vaccine;avian influenza;commercial chickens;nanovaccine
作者：Shan, SH; Fenwick, S; Ellis, T; Poinern, E; Edwards, J; Le, X; Jiang, ZT
作者单位：澳大利亚莫道克大学
期刊名称：AVIAN PATHOLOGY
期刊影响因子：1.336
出版年份：2016
出版刊次：11
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