中文摘要：文章研究了酿酒酵母葡聚糖硫酸酯(sGSC)作为佐剂的活性，体外测定了sGSC对小鼠脾淋巴细胞的作用；利用接种灭活H5N1疫苗的BALB/c小鼠体外研究了sGSC的佐剂活性。结果表明，sGSC可以显著促进淋巴细胞增殖，有效提高CD4(+) T 细胞的比例，降低CD8(+) T细胞的比例，提高CD4(+)/CD8(+)比例，增强HI抗体滴度，提高IL-2, INF-gamma, IL-4 and IL-6的浓度。这些结果表明，在小鼠模型中，葡聚糖硫酸酯是H5N1疫苗的一种有效佐剂，能够用于提高H5N1疫苗接种的免疫效应。
外文摘要：In this study, the adjuvant activity of sulfated glucan from saccharomyces cerevisiae (sGSC) was investigated. sGSC had molecular weight of 12.9 kDa and degrees of sulfation of 0.16. The effects of sGSC on the splenic lymphocyte cells of mice were measured in vitro. The results showed that sGSC could significantly promote lymphocyte proliferation singly or synergistically with Con A and LPS, and stimulate the cells to secrete IL-2 and INF-gamma. The adjuvant activity of sGSC was researched in BALB/c mice inoculated with inactivated H5N1vaccine in vivo. The results showed that sGSC could significantly enhance lymphocyte proliferation, effectively increase the percentage of CD4(+) T cells, decrease the percentage of CD8(+) T cells, and elevate the CD4(+)/CD8(+) ratio; enhance the HI antibody titre, and promote the production of IL-2, INF-gamma, IL-4 and IL-6 at medium level. These results indicated that sulfated glucan showed an excellent adjuvant effect on H5N1vaccine in a mouse model. Therefore, sGSC could be used as an effective immune adjuvant for an inactivated H5N1vaccine.
外文关键词：Sulfated glucan from saccharomyces cerevisiae; H5N1vaccine; Adjuvant activity
作者：Wang, M; Zhang, LF; Yang, RL; Fei, CZ; Wang, XY; Zhang, KY; Wang, CM; Zheng, WL; Xue, FQ
作者单位：中国农科院上海兽医研究所
期刊名称： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
期刊影响因子：3.138
出版年份：2016
出版刊次：12
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