中文摘要：研究将肿瘤自噬体与微生物HSP70多肽表位407-426（M2）耦合设计了一种增强型抗肿瘤疫苗。与单独使用肿瘤自噬体相比，新组装的疫苗无论是在皮下肿瘤模型还是在肺转移模型中都能显著抑制小鼠肺癌；耦合疫苗诱导产生的抗原特异性CTL的表达量、产生的DC2.4中CD83 和 CD86浓度更高，并提高了DC2.4中肿瘤自噬体抗原的内化水平。该研究结果表明，肿瘤自噬体-M2疫苗在临床癌症治疗方面具有很大的应用潜力。
外文摘要：Tumor-derived autophagome (DRibble) is an effective therapeutic cancer vaccine inducing T cell recognition and death of tumor cells in mice. However, the potential for improved anti-tumor response still remains. Our previous study demonstrated that two repeats of a mycobacterial HSP70(407-426) (M2) peptide acted as adjuvant in improving anti-tumor efficacy of human umbilical vein endothelial cell (HUVEC) vaccine. Here, a DRibble vaccine conjugated with M2 (DRibble-M2) was designed as a novel vaccine to enhance anti-tumor activity. Compared with DRibble alone, DRibble-M2 vaccination more significantly inhibited the growth of mouse Lewis lung cancer both in a subcutaneous tumor model and in a lung metastasis model. Higher expression of antigen-specific CTL was induced by DRibble-M2. DRibble-M2 induced higher CD83 and CD86 expression in DC2.4 and also improved the internalization of DRibble antigen into DC2.4. Our data indicated that DRibble-M2 is a potential vaccine for clinical cancer therapy.
外文关键词： Cancer immunotherapy, Autophagosome, 2mHSP70(407-426), Lung cancer KeyWords Plus: CROSS-PRESENTATION; ANTIGENS; CELLS; RESPONSES; PROTEINS; DRIBBLES
作者：Li, J; Xing, Y; Zhou, ZX; Yao, WJ; Cao, RY; Li, TM; Xu, ML; Wu, J
作者单位：中国药科大学
期刊名称：TUMOR BIOLOGY
期刊影响因子：2.926
出版年份：2016
出版刊次：11
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