中文摘要：本研究第一个目标是旨在通过BALB/c小鼠模型、以沙眼衣原体（Ct）E型多形外膜蛋白C N末端部分（N-PmpC）作为亚单位疫苗抗原分析滴眼免疫的特异性结膜免疫反应和阴道免疫反应的特征。其次是检验鼠李糖乳杆菌作为N-PmpC佐剂的特性。鼠李糖乳杆菌作为佐剂与N-PmpC联用能够激活T细胞反应，其特征是提高CD25+ T 细胞和 CD8+ 增强型T细胞比例，促进CD4+ T细胞向T helper 1细胞分化，增强调控性T细胞反应。综上所述，以N-PmpC与活性鼠李糖乳杆菌联用进行滴眼免疫能够同时激起结膜和阴道粘膜产生特异性细胞和体液免疫反应。
 
外文摘要：Recent advances in the development of chlamydia vaccines, using live-attenuated or ultraviolet light-inactivated chlamydia, are paving the way for new possibilities to oppose the societal challenges posed by chlamydia-related diseases, such as blinding trachoma. An effective subunit vaccine would mitigate the risks associated with the use of a whole-cell vaccine. Our rationale for the design of an efficient subunit vaccine against Chlamydia trachomatis (Ct) is based on the membrane proteins involved in the initial Ct-host cell contact and on the route of immunization that mimics the natural infection process (i.e., via the ocular mucosa). The first aim of our study was to characterize the specific conjunctival and vaginal immune responses following eye drop immunization in BALB/c mice, using the N-terminal portion of the Ct serovar E polymorphic membrane protein C (N-PmpC) as the subunit vaccine antigen. Second, we aimed to examine the adjuvant properties of the probiotic Lactobacillus rhamnosus (LB) when formulated with N-PmpC. N-PmpC applied alone stimulated the production of N-PmpC-and Ct serovar B-specific antibodies in serum, tears and vaginal washes, whereas the combination with LB significantly enhanced these responses. The N-PmpC/LB combination initiated a T cell response characterized by an elevated percentage of CD25+ T cells and CD8+ effector T cells, enhanced CD4+ T-helper 1 skewing, and increased regulatory T cell responses. Together, these results show that eye drop vaccination with combined use of N-PmpC and a live probiotic LB stimulates specific cellular and humoral immune responses, not only locally in the conjunctiva but also in the vaginal mucosa, which could be a promising approach in Ct vaccine development.
外文关键词：REGULATORY T-CELLS; TRACHOMATIS INFECTION; PROTECTIVE IMMUNITY; GENITAL-TRACT; VACCINE; ADHESION; DELIVERY; FAMILY; IGG
作者：Inic-Kanada, A; Stojanovic, M; Marinkovic, E; Becker, E; Stein, E; Lukic, I; Djokic, R; Schuerer, N; Hegemann, JH; Barisani-Asenbauer, T
作者单位：维也纳医科大学
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2016
出版刊次：9
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