中文摘要：本研究分析了纳洛酮/明矾混合物作为佐剂在提高人类HPV-16E7d疫苗免疫原性方面的作用。结果表明：应用纳洛酮/明矾混合物佐剂显著增强了淋巴细胞增殖，提高了Th1和Th1细胞因子的浓度。对体液免疫反应的分析表明，应用纳洛酮/明矾混合物能显著增强特异性Ig G反应和同型抗体的浓度。结论：纳洛酮/明矾混合物作为佐剂可以提高细胞性和体液免疫反应，可用于HPV疫苗模型进行人类临床试验研究。
外文摘要：Many types of human papillomaviruses (HPVs) have been identified, with some leading to cancer and others to skin lesions such as anogenital warts. Studies have demonstrated an association between oncogenic HPV and cervical cancer and many researchers have focused on therapeutic vaccines development. At present, the modulatory effect of opioids on the innate and acquired immune system is characterized. Antagonists of opioid receptors such as naloxone (NLX) can contribute to the shifting Th2 response toward Th1. Herein; we studied the adjuvant activity of NLX/Alum mixture for improvement of the immunogenicity of HPV-16E7d vaccine. Materials and Methods: The mice were administered different regimens of vaccine; E7d, E7d-NLX, E7dAlum, E7d-NLX-Alum, NLX, alum and PBS via subcutaneous route for three times with two weeks interval. Two weeks after the last immunization, the sera were assessed for total antibody, IgG1 and IgG2a with an optimized ELISA method. The splenocytes culture supernatant was analyzed by ELISA for the presence of IL-4, IFN-gamma and IL-17 cytokines and lymphocyte proliferation was evaluated with Brdu method. Results: Immunization of mice with HPV-16 E7d vaccine formulated in NLX/Alum mixture significantly increased lymphocyte proliferation and Th1 and Th17 cytokines responses compared to other experimental groups. Analysis of humoral immune responses revealed that administration of vaccine with NLX/Alum mixture significantly increased specific IgG responses and also isotypes compared to control groups. Conclusion: NLX/Alum mixture as an adjuvant could improve cellular and humoral immune responses and the adjuvant maybe useful for HPV vaccines model for further studies in human clinical trial.
外文关键词：Adjuvant; Alum; Naloxone; Papillomavirus; Vaccine
作者：Yasaghi, M; Mahdavi, M
作者单位：伊斯兰自由大学
期刊名称：Iranian Journal of Basic Medical Sciences
期刊影响因子：1.22
出版年份：2016
出版刊次：9
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