中文摘要：本发明公开了一种具有佐剂特性并可用于疫苗制备的多酚，同时公开了一种由多酚、免疫刺激重组流感病毒颗粒（IRIVs）等传递系统构成的佐剂系统。该免疫系统能够刺激机体产生更高水平的免疫反应。这类多酚主要为β-谷甾醇，β-谷甾醇能与佐剂随意结合从而增强机体免疫反应。
外文摘要：The present invention relates to certain polyphenol(s) having adjuvant property that can be used for the vaccine preparation. Also, the current invention provides adjuvant system comprising said polyphenol(s) and delivery system such as an immunostimulating reconstituted influenza virosomes (IRIVs). The present invention illustrates the said polyphenol(s) or an adjuvant system comprising of such polyphenol(s) and IRIVs can provide better level of immune response against antigen of interest than conventional vaccine systems. The preferred polyphenol according to the present invention can be beta-sitosterol. Beta-sitosterol can be optionally combined with the known adjuvant(s) to enhance immune response.
NOVELTY - Immunogenic composition comprises: (a) an antigen; and (b) beta-sitosterol as an adjuvant.
USE - The immunogenic composition is useful for preparing pharmaceutical composition and vaccine for stimulating or inducing immune response of patient against antigen, where antigen is an infectious agent selected from a bacterium, a virus, a parasite and a fungus; or the antigen is selected from leishmania, human immunodeficiency virus (HIV), hepatitis C virus (HCV), hepatitis E virus (HEV), hepatitis A virus (HAV), hepatitis B virus (HBV), tuberculosis, herpes simplex virus (HSV), malaria causing parasites, human papilloma virus (HPV), proprotein convertase subtilisin kexin 9 (PCSK9) peptide, influenza virus, measles virus, mumps virus, Ebola virus, respiratory syntial virus (RSV), West Nile virus (WNV) or its combination (all claimed).
ADVANTAGE - Provided is combination of phytosterols, pref! erably beta-sitosterol and IRIV, without destroying the immunostimulating effect of each system. The adjuvant system shows a higher stimulating effect than virosome alone.
主权项：(1) a method of preparing immunogenic composition comprising: (a) preparation of antigen; (b) formulating a modified virosome with antigen or preparing mixture comprising second adjuvant and antigen; and (c) addition of beta-sitosterol into modified virosome having antigen or into mixture comprising second adjuvant and antigen; (2) a vaccine containing immunogenic composition for inducing an immune response against antigen; (3) a method of stimulating immune response of a patient, comprising administering a suitable dosage of immunogenic composition; and
(4) a method for extracting beta-sitosterol, comprising: (a) isolation of different organs of plant system; (b) extraction of plant oil from the isolated organ; (c) extraction of the desired component preferably flavonoids or polyohenols from the extracted plant oil; and (d) separation of the beta-sitosterol from the mixture of polyphenols.
 
申请号：WO2016IB56213
公开/公告号：WO2017068482A1
申请日：2016-10-17
公开/公告日：2017-04-27
申请/专利权人：CADILA HEALTHCARE LTD (CDLA-C)
发明/设计人：FICHERA, E;GUPTA, G;GLUECK, R
分类号：A61K39/0008; A61K39/015; A61K39/12; A61K2039/55505; A61K2039/55566; A61K2039/55577; C12N2710/20023; C12N2710/20034; C12N2760/16134
主分类号：A61K39/00; A61K39/015; A61K39/12
点击下载：一种佐剂及利用该佐剂制作疫苗的方法