中文摘要：本发明通过包含融合蛋白或编码该蛋白的转染子增强动物抗原肽的免疫原性；所述的融合蛋白包括一种抗原肽和一种佐剂蛋白，佐剂蛋白由两种及两种以上蛋白组成，这些蛋白来源于志贺毒素2e B 亚单元（Stx2eB）、不耐热大肠杆菌毒素B亚单元（LTB）和霍乱毒素B亚单元（CTB）。
 
外文摘要： Immunogenicity of an antigen peptide is increased by administering, to an animal, a transfectant that has been transfected with a fusion protein or a gene that codes the same, said fusion protein including an antigen peptide and an adjuvant protein, wherein the adjuvant protein includes two or more proteins selected from the group consisting of the B subunit (Stx2eB) of Shiga toxin 2e, the B subunit (LTB) of a heat-labile Escherichia coli toxin, and the B subunit (CTB) of a cholera toxin.
NOVELTY - A fusion protein comprising (a) antigen peptide (excluding peptide derived from glycoprotein 5 (gp5) of porcine reproductive and respiratory syndrome (PRRS) virus) and an adjuvant protein, is new. The adjuvant protein is chosen from two or more proteins chosen from B subunit of Shiga toxin 2e (Stx2eB), B subunit of Escherichia coli heat-labile enterotoxin (LTB) and B subunit of cholera toxin (CTB).
USE - The fusion protein is useful for enhancing immunity in a non-human mammal (claimed) and treating anthrax, tetanus, swine erysipelas, swine dysentery, salmonellosis, colibacillosis, atrophic rhinitis, Actinobacillus diseases, mycoplasma infection, porcine transmissible gastroenteritis, porcine epidemic diarrhea, porcine influenza, Japanese encephalitis, Aujeszky's disease, foot-and-mouth disease, swine vesicular disease, hog cholera, leukem ia, genital and respiratory syndrome, rotavirus, ascariasis, toxoplasmosis, coccidiosis, hemorrhagic septicemia, brucellosis, rinderpest, epidemic encephalitis, rabies, vesicular stomatitis, rift valley disease, Johne's disease, bluetongue, Akabane disease, Chuzan disease, lumpy skin disease, bovine viral diarrhea, bovine leukemia, piroplasmosis and anaplasmosis, mycoplasmosis, avian colibacillosis, haemophilus, new castle disease, highly pathogenic avian influenza, avian infectious bronchitis, fowl pox, encephalomyelitis and tuberculosis.
ADVANTAGE - The fusion protein improves the livestock immunity and produces vaccine in a cost-effective and less labor-intensive manner, and the vaccine reduces livestock stress.
 
主权项：INDEPENDENT CLAIMS are included for the following:
(1) a DNA encoding the fusion protein;
(2) a DNA construct comprising the DNA;
(3) a recombinant vecto r comprising the DNA construct;
(4) a transformant transfo rmed with the vector; and
(5) a vaccine and an animal feed comprising the fusion protein or the transformant.
申请号：WO2016JP85606
公开/公告号：WO2017094793A1
申请日：2016-11-30
公开/公告日：2017-06-08
申请/专利权人：IDEMITSU KOSAN CO LTD (IDEK-C)
发明/设计人：SAWADA, K;MATSUI, T
分类号：A01H5/00; A23K20/195; A61K36/28; A61K39/00; C07K14/245; C07K14/28; C07K14/405; C07K19/00; C12N15/09
主分类号：A01H5/00; A23K20/195; A61K36/28; A61K39/00; A61P37/04; C07K14/245; C07K14/28; C07K14/405; C07K19/00; C12N15/09
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