中文摘要： 为了解决目前市场上缺乏高效分离和培养猪肺炎支原体的培养基，相关疫苗生产成本高等技术问题，本发明旨在提供一种猪肺炎支原体培养基及其制备方法，以及探究了其在制备相关猪肺炎支原体疫苗生产中的应用。本发明的猪肺炎支原体培养基能够快速分离和培养猪肺炎支原体，培养猪肺炎支原体的滴度可以达到10?10CCU，培养时间最低可至42h，减少了陈旧菌和老龄菌的产生，能够获得更高的疫苗免疫原性。  
外文摘要：*Mycoplasma* hyopneumoniae *culture* medium comprises basic *culture* medium and auxiliary medium, where the basic *culture* medium comprises 25-35 ml 20asteriskHank's, 2-3 g PPLO broth powder, 2-5 g yeast extract, 0.5-3 g lactalbumin hydrolysate, 3-5 g brain heart infusion powder, 0.5-1 ml of 1% zinc chloride solution, 10 ml of 0.25% phenol red solution and 850-900 ml deionized *water*, and the auxiliary medium comprises 3-4 ml of 5% arginine solution, 2-3 ml of 5% cysteine solution, 1-2 ml of 1% taurine solution, 1-5 ml of 5% coenzyme I, 5-10 ml of 100000 U/ml penicillin and 70-90 ml inactivated healthy pig serum.   INDEPENDENT CLAIMS are also included for preparing the medium, comprising (i) uniformly stirring 20asteriskHank's, PPLO broth powder, yeast extract, lactalbumin hydrolysate, brain heart infusion powder, 1% zinc chloride solution, 0.25% phenol red solution and deionized *water*, adjusting the pH to 7.2-7.4 with 10M sodium hydroxide, autoclaving at 120 degrees C for 15 minutes, cooling at room temperature, (ii) inactivating 5% arginine solution, 5% cysteine solution, 1% taurine solution, 5% coenzyme I, 100000 U/ml penicillin and healthy pig serum at 56 degrees C with cobalt-60 irradiation, and (iii) adding auxiliary medium to the cooled basal medium and uniformly mixing, and adjusting the pH to 7.6 with 1M sodium hydroxide; and #preparing *Mycoplasma* hyopneumoniae *vaccine*, rapidly separating and culturing *Mycoplasma* hyopneumoniae, where the titer of cultured *Mycoplasma* hyopneumoniae can reach 1010-1011CCU, and incubating for 42 hours.    Preferred Composition: The medium comprises basic *culture* medium and auxiliary medium, where the basic *culture* medium comprises 30 ml 20asteriskHank's, 2.5 g PPLO broth powder, 3 g yeast extract, 2 g lactalbumin hydrolysate, 4 g brain heart infusion powder, 0.75 ml of 1% zinc chloride solution, 10 ml of 0.25% phenol red solution and 870 ml deionized *water*, and the auxiliary medium comprises 3.5 ml of 5% arginine solution, 2.5 ml of 5% cysteine solution, 1 ml of 1% taurine solution, 3 ml of 5% coenzyme I, 8 ml of 100000 U/ml penicillin and 75 ml inactivated healthy pig serum.    The medium is useful for breeding pig (claimed).   The medium reduces production of stale and aged bacteria. The *vaccine* has high-*immunogenicity*.
专利号：CN201810768645.8
公开/公告号：CN109055255A
申请日：2018-07-13
公开/公告日：2018-12-21
申请/专利权人：北京农学院
发明/设计人：张永红;崔德凤;薛振华;侯佳佳
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