中文摘要：中药五味子（SC）常用于治疗各种神经系统疾病，如烦躁、焦虑、失眠、多梦等。酒制五味子（WSC）在临床上的应用已有数千年历史。本研究旨在探讨SC和WSC通过调节肠道菌群改善焦虑行为的可能机制及相关代谢。研究发现，SC和WSC均能显著改善焦虑症大鼠的焦虑、抑郁行为和脂代谢紊乱，减轻海马神经炎。经WSC治疗后，焦虑症大鼠肠道菌群结构和组成发生了显著变化，血浆和粪便中肠道菌群乳酸衍生物水平显著降低。WSC治疗有助于恢复焦虑症大鼠肠道微生物生态系统失调，逆转乳酸菌科、乳酸杆菌、异丙戊酸杆菌和类杆菌的变化。此外，SC和WSC处理的焦虑大鼠肝脏GPR81表达降低，cAMP途径分子增加。
外文摘要：Ethnopharmacological relevance: In traditional Chinese medicine, the fruit of Schisandra chinensis (Turcz.) Baill (SC) is used to treat various nervous system diseases, such as dysphoria, anxiety, insomnia and many dreams. It is worthy to be noted that wine processed Schisandra chinensis (WSC) has been applied in clinic for thousands of years.
   Aim of study: This study aimed to investigate the possible mechanism and related metabolism of SC and WSC ameliorating anxiety behavior through modulating gut microbiota.
   Materials and methods: The ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) was used for the quality control of chemical components in SC and WSC. Chronic unpredictable stress procedure (CUSP)-induced anxiety rats were administrated with SC and WSC via gavage for five weeks. An untargeted UPLC/LTQ-Orbitrap MS metabolomic analysis of plasma was conducted to understand the effects of long-term intake of WSC and SC extracts on anxious rats. 16S rRNA microbial sequencing technology was applied to investigate gut microbiota structure. Expression of GPR81, TNF-alpha, S1PR2 as well as molecules in cAMP pathway was assayed by immunohistochemistry staining, RT-qPCR, or Western blot, respectively.
   Results: 12 compounds were identified using UPLC-QTOF-MS technology, all of which are lignans. Results demonstrate that the amounts of 6-O-Benzoylgomisin O, Schisandrin, Gomisin D, Schizandrin A, Gomisin T, Schizandrin B, Schisandrin C were higher in wine-processed samples than in raw samples. Furthermore, both SC and WSC significantly ameliorated anxietyand depression-like behavior and lipid metabolism dysfunction and attenuated hippocampal neuritis in anxiety rats. After WSC treatment, the structure and composition of gut microbiota in anxiety rats changed significantly, and gut microbiota derivatives lactate level was significantly lower in the plasma and feces. WSC treatment help restore gut microbial ecosystem dysbiosis and reverse the changes in Lachnospiraceae, Lactobacillus, Alloprevotella, and Bacteroidales in anxiety rat. In addition, the expression of liver GPR81 was decreased, and the molecules in cAMP pathway were increased in SC and WSC-treated anxiety rat.
   Conclusion: Raw and wine processed Schisandra chinensis treatment improved anxietyand depression-like behavior through modulating gut microbiota derivatives in association with GPR81 receptor-mediated lipid metabolism pathway. And WSC has more exhibition than SC.
外文关键词：Gut microbiota; 16S rRNA; Schisandra chinensis; Metabonomics; Anxiety; GPR81
作者：Song, YG；Shan, BX；Zeng, SF；Zhang, J；Jin, C；Liao, Z；Wang, TT；Zeng, Q；He, HW；Wei, FQ；Ai, ZF；Su, D
作者单位：Jiangxi Univ Tradit Chinese Med；China Pharmaceut Univ；Qingdao Municipal Hosp Grp
期刊名称：JOURNAL OF ETHNOPHARMACOLOGY
期刊影响因子：3.69
出版年份：2021
出版刊次：266
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