中文摘要：全球食源性疾病的持续报道和耐药细菌的广泛流行，对食品安全和人类健康造成了极大的威胁，迫切需要新型杀菌、控菌技术的研发。噬菌体裂解酶是大部分裂性噬菌体在裂解期释放一种活性蛋白，能够有效裂解宿主细胞壁,已被证明可在食品供应链的各个环节中控制食源性致病菌风险。天然噬菌体裂解酶具有高度的宿主特异性和强烈的裂解活性，并能破坏细菌生物被膜，而且具备绿色安全、不易产生耐药等优势。同时，噬菌体裂解酶具有模块化结构特点，运用蛋白质工程技术将其重组，可增强其裂解活性、提高稳定性以及靶向性。本综述系统地描述了噬菌体裂解酶的模块化结构特征及作用位点，讨论了噬菌体裂解酶的重组策略和方法,总结了天然噬菌体裂解酶在控制食源性致病菌方面的应用进展,并对噬菌体裂解酶在食品工业中的应用进行了展望，以期为食源性致病菌及其耐药性的有效控制提供一种行之有效的新技术。
外文摘要：The continuous reports of foodborne diseases and the widespread prevalence of antimicrobial-resistance bacteria have posed a great threat to food safety and human health. And the research as well as development of new bactericidal technologies is urgently needed. Phage lysins are active proteins released by most lytic phages in the lytic period that can effectively lyse the cell wall of the host and have been shown to control the risk of foodborne pathogens in all links of the chain of farm-to-table. Natural lysins, with high host specificity, strong lytic activity, green security and other advantages, are capable of eradicating the biofilm. Importantly, lysins have modular structures that can be recombined by protein engineering to enhance their cleavage activity, stability and targeting. This review systematically described the modular structural characteristics and activity sites of phage lysins, discussed the recombination strategies of lysins and summarized the progress of natural lysin to control foodborne pathogens. And also, the application of lysins in food industry was prospected. This review aims to provide novel technologies and strategies to control foodborne pathogens and their antimicrobial-resistance.
中文关键词：噬菌体裂解酶；食源性致病菌；耐药性；结构特征；重组策略
外文关键词：phage lysins; foodborne pathogens; antimicrobial resistance (AMR); structure characteristics; recombination strategies
作者：黄振华；张昭寰；童金蓉；吴 倩；刘 静；刘海泉；潘迎捷；赵 勇
作者单位：上海海洋大学食品学院；上海海洋大学水产与生命学院；农业农村 部（上海）水产品贮藏保鲜质量安全风险评估实验室；上海水产品加工及贮藏工程技术研究中心
期刊名称：食品科学
期刊影响因子：2.115
出版年份：2020
出版刊次：
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