中文摘要：本研究旨在探讨壳寡糖（COS）通过调节肠道微生物群对便秘的改善作用。研究表明，对于由洛哌丁胺引起的便秘小鼠，COS治疗能显著促其进肠道运动，抑制炎症反应，改善水-电解质代谢，防止肠道屏障损伤。通过16S rDNA基因测序，发现便秘小鼠的肠道菌群失衡；而通过计数发现，用COS治疗逆转了几种肠道细菌在门、科和属水平上的丰度变化，在一定程度上维持了其胆汁酸、短链脂肪酸和色氨酸等肠道代谢物的平衡。此外，COS未能缓解肠道菌群耗竭的小鼠便秘症状，证实了肠道菌群在COS预防便秘中的重要性。
外文摘要：This study was designed to explore the improvement of chitosan oligosaccharides (COS) on constipation through regulation of gut microbiota. Here, we proved that COS treatment profoundly boosted intestinal motility, restrained inflammatory responses, improved water-electrolyte metabolism and prevented gut barrier damage in constipated mice induced by loperamide. By 16S rDNA gene sequencing, the disbalanced gut microbiota was observed in constipated mice, while COS treatment statistically reversed the abundance changes of several intestinal bacteria at either phylum, family and genus levels, which partly led to the balance in production of intestinal metabolites including bile acids, short-chain fatty acids and tryptophan catabolites. In addition, COS failed to relieve the constipation in mice with intestinal flora depletion, confirming the essentiality of gut microbiota in COS-initiated prevention against constipation. In summary, COS can ameliorate the development of loperamide-induced constipation in mice by remodeling the structure of gut microbial community.
外文关键词：Chitosan oligosaccharides (COS); Constipation; Gut microbiota; Short chain fatty acids (SCFAs); Bile acids (BAs)
作者：Zhang, XY；Yang, HB；Zheng, JP；Jiang, N；Sun, GJ；Bao, XK；Lin, AZ；Liu, HT
作者单位：Hubei Univ Chinese Med；Hubei Prov Hosp Tradit Chinese Med；Hubei Prov Acad Tradit Chinese Med；Chongqing Acad Chinese Mat Medica
期刊名称：CARBOHYDRATE POLYMERS
期刊影响因子：7.182
出版年份：2021
出版刊次：253
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