中文摘要：近年来，抗生素污染已成为全世界面临的严重公共卫生问题，光动力灭活可以避免抗生素滥用，并最大限度地降低抗生素耐药性风险，是抗菌处理中最具前景的创新方法之一。但是，由于光敏剂与革兰氏阴性菌之间的相互作用较弱，难以充分发挥光动力灭活作用。为此构建了含氯聚乙烯亚胺纳米粒子的光敏剂。纳米粒子与细菌表面的静电和疏水相互作用，协同促进了纳米粒子在细菌表面的固定作用，增强了对革兰氏阴性细菌的光动力灭活活性。研究发现，其对革兰氏阳性细菌金黄色葡萄球菌也有显著的抗菌作用。细胞摄取实验表明，采用支化聚乙烯亚胺纳米粒子，可以将光敏剂牢固地固定在大肠杆菌或金黄色葡萄球菌细胞的表面；与膜结合的纳米粒子在光触发下产生活性氧，对细菌外膜造成不可逆的损伤，实现了比游离光敏剂更高的杀菌效率。该研究将提供一种有效且前景很好的抗菌素替代品，以防止抗生素的过度使用，并在人类健康及环境修复方面具有巨大的潜力。
外文摘要：Antibiotic pollution has been a serious global public health concern in recent years, photodynamic inactivation is one of the most promising and innovative methods for antibacterial applications that avoids antibiotic abuse and minimizes risks of antibiotic resistance. However, limited by the weak interaction between the photosensitizers and Gram-negative bacteria, the effect of photodynamic inactivation cannot be fully exerted. Herein, photosensitizer chlorine-loaded polyethyleneimine-based micelle was constructed. The synergy of electrostatic and hydrophobic interactions between the nanoparticles and the bacterial surface promoted the anchoring of nanoparticles onto the bacteria, resulting in enhanced photoinactivation activities on Gram-negative bacteria. As expected, an eminent antibacterial effect was also observed on the Gram-positive bacteria Staphylococcus aureus. The cellular uptake results showed that photosensitizer was firmly anchored to the bacterial cell surface of Escherichia coli or Staphylococcus aureus by the introduction of branched polyethylenimine-containing nanoparticles. The light-triggered generation of reactive oxygen species, mainly singlet oxygen, from the membrane bound nanoparticles caused irreversible damage to the bacterial outer membrane, achieving enhanced bactericidal efficiency than free photosensitizer. The study would provide an efficient and promising antimicrobial alternative to prevent overuse of antibiotics and have enormous potential for human healthcare and the environment remediation.
外文关键词：Photodynamic inactivation; Porphyrin; Antibacterial; Pathogenic bacteria
作者：Wang, Q；Zhang, DD；Feng, J；Sun, TL；Li, CL；Xie, XB；Shi, QS
作者单位：Guangdong Acad Sci
期刊名称：JOURNAL OF COLLOID AND INTERFACE SCIENCE
期刊影响因子：7.489
出版年份：2021
出版刊次：584
点击下载：可见光照射下支化聚乙烯亚胺纳米粒子能增强革兰氏阴性菌的光动力灭活作用